@article{f66d8cfc48f4424d8b4cffd834ea772a,
title = "Yng1 PHD Finger Binding to H3 Trimethylated at K4 Promotes NuA3 HAT Activity at K14 of H3 and Transcription at a Subset of Targeted ORFs",
abstract = "Posttranslational histone modifications participate in modulating the structure and function of chromatin. Promoters of transcribed genes are enriched with K4 trimethylation and hyperacetylation on the N-terminal tail of histone H3. Recently, PHD finger proteins, like Yng1 in the NuA3 HAT complex, were shown to interact with H3K4me3, indicating a biochemical link between K4 methylation and hyperacetylation. By using a combination of mass spectrometry, biochemistry, and NMR, we detail the Yng1 PHD-H3K4me3 interaction and the importance of NuA3-dependent acetylation at K14. Furthermore, genome-wide ChIP-Chip analysis demonstrates colocalization of Yng1 and H3K4me3 in vivo. Disrupting the K4me3 binding of Yng1 altered K14ac and transcription at certain genes, thereby demonstrating direct in vivo evidence of sequential trimethyl binding, acetyltransferase activity, and gene regulation by NuA3. Our data support a general mechanism of transcriptional control through which histone acetylation upstream of gene activation is promoted partially through availability of H3K4me3, {"}read{"} by binding modules in select subunits.",
keywords = "DNA",
author = "Taverna, {Sean D.} and Serge Ilin and Rogers, {Richard S.} and Tanny, {Jason C.} and Heather Lavender and Haitao Li and Lindsey Baker and John Boyle and Blair, {Lauren P.} and Chait, {Brian T T.} and Patel, {Dinshaw J.} and Aitchison, {John D.} and Tackett, {Alan J.} and Allis, {C. David}",
note = "Funding Information: All FITC-labeled and most biotin-labeled peptides were generated by N. Chandramouli and S. Powell at the Proteomics Resource Center of The Rockefeller University. Histone antibodies from UBI/Serologicals were a gift from the company. A.J.T. thanks C. Warthen for MS support from AR INBRE (P20RR016460), and AR Biosciences Inst. We thank Allis laboratory members for helpful discussions, especially E. Goneska and Drs. A. Ruthenburg, Y. Dou, and T. Milne for critical reading of this manuscript. A.J.T. acknowledges insightful discussions with Drs. W. Wahls and K. Raney. This work was supported by NIH grants to S.D.T. (GM53512), L.B., (CA09673), C.D.A. (GM63959), A.J.T. (P20RR015569), J.D.A. (RR022220 and GM076547), and B.T.C. (RR00862 and RR022220) as well as Rockefeller University. D.J.P. is supported by funds from the Abby Rockefeller Mauze Trust, Dewitt Wallace and Maloris Foundations, and the NIH and acknowledges generous access to the New York Structural Biology Center's NMR facilities. ",
year = "2006",
month = dec,
day = "8",
doi = "10.1016/j.molcel.2006.10.026",
language = "English (US)",
volume = "24",
pages = "785--796",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "5",
}