Yeast STE 14 methyltransferase, expressed as TrpE-STE 14 fusion protein in Escherichia coli, for in Vitro Carboxylmethylation of prenylated polypeptides

Christine A. Hrycyna; Stephanie J. Wait; Peter S. Backlund; Susan Michaelis

doi:10.1016/0076-6879(95)50077-4

Yeast STE 14 methyltransferase, expressed as TrpE-STE 14 fusion protein in Escherichia coli, for in Vitro Carboxylmethylation of prenylated polypeptides

Christine A. Hrycyna, Stephanie J. Wait, Peter S. Backlund, Susan Michaelis

School of Medicine

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Original language	English (US)
Pages (from-to)	251-266
Number of pages	16
Journal	Methods in enzymology
Volume	250
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(95)50077-4
State	Published - Jan 1 1995

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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@article{f3cb090c0ccf4a749937c4110e62839b,

title = "Yeast STE 14 methyltransferase, expressed as TrpE-STE 14 fusion protein in Escherichia coli, for in Vitro Carboxylmethylation of prenylated polypeptides",

author = "Hrycyna, {Christine A.} and Wait, {Stephanie J.} and Backlund, {Peter S.} and Susan Michaelis",

note = "Funding Information: We are indebted to D. S. King for peptide synthesis, mass spectrometry, and unending technical support. We thank L. Wood and A. Moser (University of California, San Francisco) for rat tissues and R. Kim and S.-H. Kim for Xenopus oocyte samples and many helpful discussions. We also thank S. Rosenberg and C. C. Yang (Protos, Emeryville, CA) for dan-sylated peptides and members of the laboratory of J. R. for insightful discussions during the course of the experiments. The work was supported by the California Tobacco-Related Disease Research Program (2FT0030 to M. N. A. and IRT26 to J. R.) and by a National Institute of Environmental Health Sciences Mutagenesis Center grant (P30 ESO 1896). Funding Information: We thank Steve Clarke in whose laboratory part of this work was carried out (by C. A. H.) and Kathleen Green Vancura for technical assistance in construction of trpE-STE14 gene fusions. We also thank Carol Berkower for helpful comments on the manuscript. This work was supported by grants from the National Institutes of Health to Steve Clarke (GM26020) and to S. M. (GM41223). C. A. H. was supported in part by a U.S. Public Health Service Training Grant (GM07185).",

year = "1995",

month = jan,

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doi = "10.1016/0076-6879(95)50077-4",

language = "English (US)",

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T1 - Yeast STE 14 methyltransferase, expressed as TrpE-STE 14 fusion protein in Escherichia coli, for in Vitro Carboxylmethylation of prenylated polypeptides

AU - Hrycyna, Christine A.

AU - Wait, Stephanie J.

AU - Backlund, Peter S.

AU - Michaelis, Susan

N1 - Funding Information: We are indebted to D. S. King for peptide synthesis, mass spectrometry, and unending technical support. We thank L. Wood and A. Moser (University of California, San Francisco) for rat tissues and R. Kim and S.-H. Kim for Xenopus oocyte samples and many helpful discussions. We also thank S. Rosenberg and C. C. Yang (Protos, Emeryville, CA) for dan-sylated peptides and members of the laboratory of J. R. for insightful discussions during the course of the experiments. The work was supported by the California Tobacco-Related Disease Research Program (2FT0030 to M. N. A. and IRT26 to J. R.) and by a National Institute of Environmental Health Sciences Mutagenesis Center grant (P30 ESO 1896). Funding Information: We thank Steve Clarke in whose laboratory part of this work was carried out (by C. A. H.) and Kathleen Green Vancura for technical assistance in construction of trpE-STE14 gene fusions. We also thank Carol Berkower for helpful comments on the manuscript. This work was supported by grants from the National Institutes of Health to Steve Clarke (GM26020) and to S. M. (GM41223). C. A. H. was supported in part by a U.S. Public Health Service Training Grant (GM07185).

PY - 1995/1/1

Y1 - 1995/1/1

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AN - SCOPUS:0029022640

SN - 0076-6879

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JO - Methods in enzymology

JF - Methods in enzymology

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ER -

Yeast STE 14 methyltransferase, expressed as TrpE-STE 14 fusion protein in Escherichia coli, for in Vitro Carboxylmethylation of prenylated polypeptides

ASJC Scopus subject areas

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