Yeast RNA-binding protein Nab3 regulates genes involved in nitrogen metabolism

Jonathan Merran, Jeffry L. Corden

Research output: Contribution to journalArticlepeer-review

Abstract

Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion.

Original languageEnglish (US)
Article numbere00154-17
JournalMolecular and cellular biology
Volume37
Issue number18
DOIs
StatePublished - Sep 1 2017

Keywords

  • Nitrogen metabolism
  • Noncoding RNA
  • Termination
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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