Yeast RNA-binding protein Nab3 regulates genes involved in nitrogen metabolism

Jonathan Merran, Jeffry Lynn Corden

Research output: Contribution to journalArticle

Abstract

Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion.

Original languageEnglish (US)
Article numbere00154-17
JournalMolecular and Cellular Biology
Volume37
Issue number18
DOIs
StatePublished - Sep 1 2017

Fingerprint

Fungal Proteins
RNA-Binding Proteins
Nitrogen
Exosomes
Untranslated RNA
Messenger RNA
Polyadenylation
RNA Polymerase II
RNA Stability
Genes
Saccharomyces cerevisiae

Keywords

  • Nitrogen metabolism
  • Noncoding RNA
  • Termination
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Yeast RNA-binding protein Nab3 regulates genes involved in nitrogen metabolism. / Merran, Jonathan; Corden, Jeffry Lynn.

In: Molecular and Cellular Biology, Vol. 37, No. 18, e00154-17, 01.09.2017.

Research output: Contribution to journalArticle

@article{adceee546ebd4fc19a69fb4182f8b956,
title = "Yeast RNA-binding protein Nab3 regulates genes involved in nitrogen metabolism",
abstract = "Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion.",
keywords = "Nitrogen metabolism, Noncoding RNA, Termination, Transcription",
author = "Jonathan Merran and Corden, {Jeffry Lynn}",
year = "2017",
month = "9",
day = "1",
doi = "10.1128/MCB.00154-17",
language = "English (US)",
volume = "37",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "18",

}

TY - JOUR

T1 - Yeast RNA-binding protein Nab3 regulates genes involved in nitrogen metabolism

AU - Merran, Jonathan

AU - Corden, Jeffry Lynn

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion.

AB - Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion.

KW - Nitrogen metabolism

KW - Noncoding RNA

KW - Termination

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=85028363356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028363356&partnerID=8YFLogxK

U2 - 10.1128/MCB.00154-17

DO - 10.1128/MCB.00154-17

M3 - Article

C2 - 28674185

AN - SCOPUS:85028363356

VL - 37

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 18

M1 - e00154-17

ER -