Yeast carboxyl-terminal domain kinase I positively and negatively regulates RNA polymerase II carboxyl-terminal domain phosphorylation

Meera Patturajan, Nicholas K. Conrad, David B. Bregman, Jeffry Lynn Corden

Research output: Contribution to journalArticle

Abstract

Monoclonal antibodies that recognize specific carboxyl-terminal domain (CTD) phosphoepitopes were used to examine CTD phosphorylation in yeast cells lacking carboxyl-terminal domain kinase I (CTDK-I). We show that deletion of the kinase subunit CTK1 results in an increase in phosphorylation of serine in position 5 (Ser5) of the CTD repeat (Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6- Ser7) during logarithmic growth. This result indicates that CTDK-I negatively regulates CTD Ser5 phosphorylation. We also show that CTK1 deletion (ctk1Δ) eliminates the transient increase in CTD serine 2 (Ser2) phosphorylation observed during the diauxic shift. This result suggests that CTDK-I may play a direct role in phosphorylating CTD Ser2 in response to nutrient depletion. Northern blot analysis was used to show that genes normally induced during the diauxic shift are not properly induced in a ctk1Δ strain. Glycogen synthase (GSY2) and cytosolic catalase (CTT1) mRNA levels increase about 10-fold in wild-type cells, but this increase is not observed in ctk1Δ cells suggesting that increased message levels may require Ser2 phosphorylation. Heat shock also induces Ser2 phosphorylation, but we show here that this change in CTD modification and an accompanying induction of heat shock gene expression is independent of CTDK-I. The observation that SSA3/SSA4 expression is increased in ctk1Δ cells grown at normal temperature suggests a possible role for CTDK-I in transcription repression. We discuss several possible positive and negative roles for CTDK-I in regulating CTD phosphorylation and gene expression.

Original languageEnglish (US)
Pages (from-to)27823-27828
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number39
DOIs
StatePublished - Sep 24 1999

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Phosphorylation
RNA Polymerase II
Yeast
Serine
Phosphotransferases
Yeasts
Gene expression
Shock
Hot Temperature
Cells
Gene Expression
Glycogen Synthase
Terminal Repeat Sequences
Transcription
Northern Blotting
Catalase
Nutrients
Genes
Monoclonal Antibodies
Food

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yeast carboxyl-terminal domain kinase I positively and negatively regulates RNA polymerase II carboxyl-terminal domain phosphorylation. / Patturajan, Meera; Conrad, Nicholas K.; Bregman, David B.; Corden, Jeffry Lynn.

In: Journal of Biological Chemistry, Vol. 274, No. 39, 24.09.1999, p. 27823-27828.

Research output: Contribution to journalArticle

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