Yeast as a model system for studying endocytosis

Jonathan D. Shaw, Kellie B. Cummings, Gregory Huyer, Susan Michaelis, Beverly Wendland

Research output: Contribution to journalArticle

Abstract

Endocytosis is the membrane trafficking process by which plasma membrane components and extracellular material are internalized into cytoplasmic vesicles and delivered to early and late endosomes, eventually either recycling back to the plasma membrane or arriving at the lysosome/vacuole. The budding yeast Saccharomyces cerevisiae has proven to be an invaluable system for identifying proteins involved in endocytosis and elucidating the mechanisms underlying internalization and postinternalization events. Through genetic studies in yeast and biochemical studies in mammalian cells, it has become apparent that multiple cellular processes are linked to endocytosis, including actin cytoskeletal dynamics, ubiquitylation, lipid modification, and signal transduction. In this review, we will highlight the most exciting recent findings in the field of yeast endocytosis. Specifically, we will address the involvement of the actin cytoskeleton in internalization, the role of ubiquitylation as a regulator of multiple steps of endocytosis in yeast, and the sorting of endocytosed proteins into the recycling and vacuolar pathways.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalExperimental cell research
Volume271
Issue number1
DOIs
StatePublished - Nov 15 2001

Keywords

  • Actin
  • EH domain
  • Endocytosis
  • Endosome
  • Multivesicular body
  • Protein trafficking
  • Recycling
  • Ubiquitin
  • Yeast

ASJC Scopus subject areas

  • Cell Biology

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