TY - JOUR
T1 - Yeast artificial chromosome modification and manipulation
AU - Reeves, Roger H.
AU - Pavan, William J.
AU - Hieter, Philip
PY - 1992/1/1
Y1 - 1992/1/1
N2 - This chapter describes yeast artificial chromosome (YAC) modification and manipulation. Two types of YAC modification systems have been developed that take advantage of this property to target recombination to highly repetitive DNA elements within YAC DNA inserts. Vectors that integrate into YACs can be used to produce chromosome-specific libraries from somatic cell hybrids or to introduce deletions, mutations, or mammalian selectable markers into YAC cloned segments. A second vector system facilitates analysis of the large inserts in YACs by fragmenting them, generating nested deletion derivatives. A polyethylene glycol-mediated fusion procedure has been described that can be used to introduce appropriately modified YACs into cultured mammalian cells. This combination of vectors and procedures provides a system for realizing the full potential of YACs in structural and biological analysis of the genome.
AB - This chapter describes yeast artificial chromosome (YAC) modification and manipulation. Two types of YAC modification systems have been developed that take advantage of this property to target recombination to highly repetitive DNA elements within YAC DNA inserts. Vectors that integrate into YACs can be used to produce chromosome-specific libraries from somatic cell hybrids or to introduce deletions, mutations, or mammalian selectable markers into YAC cloned segments. A second vector system facilitates analysis of the large inserts in YACs by fragmenting them, generating nested deletion derivatives. A polyethylene glycol-mediated fusion procedure has been described that can be used to introduce appropriately modified YACs into cultured mammalian cells. This combination of vectors and procedures provides a system for realizing the full potential of YACs in structural and biological analysis of the genome.
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U2 - 10.1016/0076-6879(92)16051-K
DO - 10.1016/0076-6879(92)16051-K
M3 - Article
C2 - 1336105
AN - SCOPUS:0027012625
VL - 216
SP - 584
EP - 603
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -