YAP and TAZ regulate cell volume

Nicolas A. Perez-Gonzalez, Nash D. Rochman, Kai Yao, Jiaxiang Tao, Minh Tam Tran Le, Shannon Flanary, Lucia Sablich, Ben Toler, Eliana Crentsil, Felipe Takaesu, Bram Lambrus, Jessie Huang, Vivian Fu, Pragati Chengappa, Tia M. Jones, Andrew J. Holland, Steven An, Denis Wirtz, Ryan J. Petrie, Kun Liang GuanSean X. Sun

Research output: Contribution to journalArticle

Abstract

How mammalian cells regulate their physical size is currently poorly understood, in part due to the difficulty in accurately quantifying cell volume in a high-throughput manner. Here, using the fluorescence exclusion method, we demonstrate that the mechanosensitive transcriptional regulators YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif) are regulators of single-cell volume. The role of YAP/TAZ in volume regulation must go beyond its influence on total cell cycle duration or cell shape to explain the observed changes in volume. Moreover, for our experimental conditions, volume regulation by YAP/TAZ is independent of mTOR. Instead, we find that YAP/TAZ directly impacts the cell division volume, and YAP is involved in regulating intracellular cytoplasmic pressure. Based on the idea that YAP/TAZ is a mechanosensor, we find that inhibiting myosin assembly and cell tension slows cell cycle progression from G1 to S. These results suggest that YAP/TAZ may be modulating cell volume in combination with cytoskeletal tension during cell cycle progression.

Original languageEnglish (US)
Pages (from-to)3472-3488
Number of pages17
JournalThe Journal of cell biology
Volume218
Issue number10
DOIs
StatePublished - Oct 7 2019

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Cell Size
Proteins
Cell Cycle
Cell Shape
Myosins
Cell Division
Fluorescence
Pressure

ASJC Scopus subject areas

  • Cell Biology

Cite this

Perez-Gonzalez, N. A., Rochman, N. D., Yao, K., Tao, J., Le, M. T. T., Flanary, S., ... Sun, S. X. (2019). YAP and TAZ regulate cell volume. The Journal of cell biology, 218(10), 3472-3488. https://doi.org/10.1083/jcb.201902067

YAP and TAZ regulate cell volume. / Perez-Gonzalez, Nicolas A.; Rochman, Nash D.; Yao, Kai; Tao, Jiaxiang; Le, Minh Tam Tran; Flanary, Shannon; Sablich, Lucia; Toler, Ben; Crentsil, Eliana; Takaesu, Felipe; Lambrus, Bram; Huang, Jessie; Fu, Vivian; Chengappa, Pragati; Jones, Tia M.; Holland, Andrew J.; An, Steven; Wirtz, Denis; Petrie, Ryan J.; Guan, Kun Liang; Sun, Sean X.

In: The Journal of cell biology, Vol. 218, No. 10, 07.10.2019, p. 3472-3488.

Research output: Contribution to journalArticle

Perez-Gonzalez, NA, Rochman, ND, Yao, K, Tao, J, Le, MTT, Flanary, S, Sablich, L, Toler, B, Crentsil, E, Takaesu, F, Lambrus, B, Huang, J, Fu, V, Chengappa, P, Jones, TM, Holland, AJ, An, S, Wirtz, D, Petrie, RJ, Guan, KL & Sun, SX 2019, 'YAP and TAZ regulate cell volume', The Journal of cell biology, vol. 218, no. 10, pp. 3472-3488. https://doi.org/10.1083/jcb.201902067
Perez-Gonzalez NA, Rochman ND, Yao K, Tao J, Le MTT, Flanary S et al. YAP and TAZ regulate cell volume. The Journal of cell biology. 2019 Oct 7;218(10):3472-3488. https://doi.org/10.1083/jcb.201902067
Perez-Gonzalez, Nicolas A. ; Rochman, Nash D. ; Yao, Kai ; Tao, Jiaxiang ; Le, Minh Tam Tran ; Flanary, Shannon ; Sablich, Lucia ; Toler, Ben ; Crentsil, Eliana ; Takaesu, Felipe ; Lambrus, Bram ; Huang, Jessie ; Fu, Vivian ; Chengappa, Pragati ; Jones, Tia M. ; Holland, Andrew J. ; An, Steven ; Wirtz, Denis ; Petrie, Ryan J. ; Guan, Kun Liang ; Sun, Sean X. / YAP and TAZ regulate cell volume. In: The Journal of cell biology. 2019 ; Vol. 218, No. 10. pp. 3472-3488.
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AU - Perez-Gonzalez, Nicolas A.

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AU - Yao, Kai

AU - Tao, Jiaxiang

AU - Le, Minh Tam Tran

AU - Flanary, Shannon

AU - Sablich, Lucia

AU - Toler, Ben

AU - Crentsil, Eliana

AU - Takaesu, Felipe

AU - Lambrus, Bram

AU - Huang, Jessie

AU - Fu, Vivian

AU - Chengappa, Pragati

AU - Jones, Tia M.

AU - Holland, Andrew J.

AU - An, Steven

AU - Wirtz, Denis

AU - Petrie, Ryan J.

AU - Guan, Kun Liang

AU - Sun, Sean X.

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