TY - JOUR
T1 - XSynapse location during growth depends on glia location
AU - Shao, Zhiyong
AU - Watanabe, Shigeki
AU - Christensen, Ryan
AU - Jorgensen, Erik M.
AU - Colón-Ramos, Daniel A.
N1 - Funding Information:
Some illustrations were created by Z.F. Altun and D.H. Hall from WormAtlas. The EM in Fig 5A, Fig S1C and S1D were prepared by J. White, E. Southgate, N. Thomson and S. Brenner at the LMB/MRC labs in Cambridge, England (White et al., 1986). The adult image in the graphical abstract was created by Dr. Maria Gallegos of Cal State University. We thank Dr. H. Bülow, Dr. M. Stern, Dr. S. Shaham, Dr. T. Kinnunen, Dr. J. Fares, Dr. K. Shen, and CGC for strains and reagents. We also thank A. Pérez and A. Roque for technical assistance, Dr. X. Song for helping with the protein blots, and D. Hall, S. Margolis, P. De Camilli, S. Strittmatter, M. Hammarlund, and members of the Colón-Ramos lab for helpful discussions and sharing of advice. This work was funded by the following grants to D.C.-R. (R00 NS057931, R01 NS076558, a fellowship from the Klingenstein Foundation and the Alfred P. Sloan Foundation and a March of Dimes Research Grant) and to E.M.J (NIH R01 NS034307, NSF 0920069). E.M.J. is an Investigator of the Howard Hughes Medical Institute. Z.S. and D.A.C-R. designed experiments. S.W. and E.M.J. performed the fEM experiments. R. C. performed wild type EM reconstruction and AIY Zone 1 length quantification. Z.S. performed all other experiments. Z.S. and D.A.C-R. analyzed and interpreted the data. Z.S., S.W., E.M.J., and D.A.C.-R. wrote the paper.
PY - 2013/7/18
Y1 - 2013/7/18
N2 - Synaptic contacts are largely established during embryogenesis and are then maintained during growth. To identify molecules involved in this process, we conducted a forward genetic screen in C. elegans and identified cima-1. In cima-1 mutants, synaptic contacts are correctly established during embryogenesis, but ectopic synapses emerge during postdevelopmental growth. cima-1 encodes a solute carrier in the SLC17 family of transporters that includes sialin, a protein that when mutated in humans results in neurological disorders. cima-1 does not function in neurons but rather functions in the nearby epidermal cells to correctly position glia during postlarval growth. Our findings indicate that CIMA-1 antagonizes the FGF receptor (FGFR), and does so most likely by inhibiting FGFR's role in epidermal-glia adhesion rather than signaling. Our data suggest that epidermal-glia crosstalk, in this case mediated by a transporter and the FGF receptor, is vital to preserve embryonically derived circuit architecture during postdevelopmental growth. PaperFlick
AB - Synaptic contacts are largely established during embryogenesis and are then maintained during growth. To identify molecules involved in this process, we conducted a forward genetic screen in C. elegans and identified cima-1. In cima-1 mutants, synaptic contacts are correctly established during embryogenesis, but ectopic synapses emerge during postdevelopmental growth. cima-1 encodes a solute carrier in the SLC17 family of transporters that includes sialin, a protein that when mutated in humans results in neurological disorders. cima-1 does not function in neurons but rather functions in the nearby epidermal cells to correctly position glia during postlarval growth. Our findings indicate that CIMA-1 antagonizes the FGF receptor (FGFR), and does so most likely by inhibiting FGFR's role in epidermal-glia adhesion rather than signaling. Our data suggest that epidermal-glia crosstalk, in this case mediated by a transporter and the FGF receptor, is vital to preserve embryonically derived circuit architecture during postdevelopmental growth. PaperFlick
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U2 - 10.1016/j.cell.2013.06.028
DO - 10.1016/j.cell.2013.06.028
M3 - Article
C2 - 23870123
AN - SCOPUS:84880559473
SN - 0092-8674
VL - 154
SP - 337
JO - Cell
JF - Cell
IS - 2
ER -