XRCC1 protects against the lethality of induced oxidative DNA damage in nondividing neural cells

Avanti Kulkarni, Daniel R. McNeill, Marc Gleichmann, Mark P. Mattson, David M. Wilson

Research output: Contribution to journalArticle

Abstract

XRCC1 is a critical scaffold protein that orchestrates efficient single-strand break repair (SSBR). Recent data has found an association of XRCC1 with proteins causally linked to human spinocerebellar ataxias - aprataxin and tyrosyl-DNA phosphodiesterase 1 - implicating SSBR in protection against neuronal cell loss and neurodegenerative disease. We demonstrate herein that shRNA lentiviral-mediated XRCC1 knockdown in human SH-SY5Y neuroblastoma cells results in a largely selective increase in sensitivity of the nondividing (i.e. terminally differentiated) cell population to the redox-cycling agents, menadione and paraquat; this reduced survival was accompanied by an accumulation of DNA strand breaks. Using hypoxanthine-xanthine oxidase as the oxidizing method, XRCC1 deficiency affected both dividing and nondividing SH-SY5Y cells, with a greater effect on survival seen in the former case, suggesting that the spectrum of oxidative DNA damage created dictates the specific contribution of XRCC1 to cellular resistance. Primary XRCC1 heterozygous mouse cerebellar granule cells exhibit increased strand break accumulation and reduced survival due to increased apoptosis following menadione treatment. Moreover, knockdown of XRCC1 in primary human fetal brain neurons leads to enhanced sensitivity to menadione, as indicated by increased levels of DNA strand breaks relative to control cells. The cumulative results implicate XRCC1, and more broadly SSBR, in the protection of nondividing neuronal cells from the genotoxic consequences of oxidative stress.

Original languageEnglish (US)
Pages (from-to)5111-5121
Number of pages11
JournalNucleic Acids Research
Volume36
Issue number15
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

DNA Damage
Vitamin K 3
DNA Breaks
Survival
Spinocerebellar Ataxias
Paraquat
Xanthine Oxidase
Neuroblastoma
Neurodegenerative Diseases
Small Interfering RNA
Oxidation-Reduction
Proteins
Oxidative Stress
Apoptosis
Neurons
Brain
Population

ASJC Scopus subject areas

  • Genetics

Cite this

Kulkarni, A., McNeill, D. R., Gleichmann, M., Mattson, M. P., & Wilson, D. M. (2008). XRCC1 protects against the lethality of induced oxidative DNA damage in nondividing neural cells. Nucleic Acids Research, 36(15), 5111-5121. https://doi.org/10.1093/nar/gkn480

XRCC1 protects against the lethality of induced oxidative DNA damage in nondividing neural cells. / Kulkarni, Avanti; McNeill, Daniel R.; Gleichmann, Marc; Mattson, Mark P.; Wilson, David M.

In: Nucleic Acids Research, Vol. 36, No. 15, 2008, p. 5111-5121.

Research output: Contribution to journalArticle

Kulkarni, A, McNeill, DR, Gleichmann, M, Mattson, MP & Wilson, DM 2008, 'XRCC1 protects against the lethality of induced oxidative DNA damage in nondividing neural cells', Nucleic Acids Research, vol. 36, no. 15, pp. 5111-5121. https://doi.org/10.1093/nar/gkn480
Kulkarni, Avanti ; McNeill, Daniel R. ; Gleichmann, Marc ; Mattson, Mark P. ; Wilson, David M. / XRCC1 protects against the lethality of induced oxidative DNA damage in nondividing neural cells. In: Nucleic Acids Research. 2008 ; Vol. 36, No. 15. pp. 5111-5121.
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