Xenognosin sensing in virulence: Is there a phenol receptor in Agrobacterium tumefaciens?

A. M. Campbell, J. B. Tok, J. Zhang, Y. Wang, M. Stein, D. G. Lynn, A. N. Binns

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Background: The mechanisms of signal perception and transmission in the 'two-component' autokinase transmitters/response regulators are poorly understood, especially considering the vast number of such systems now known. Virulence induction from the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens represents one of the best understood systems with regard to the chemistry of the activating signal, and yet the existing data does not support a receptor-mediated perception event for the xenognostic phenols. Results: Here we provide the first conclusive evidence that a specific receptor must be involved in xenognostic phenol perception, detail structural requirements of the xenognosins necessary for perception by this receptor, and develop a genetic strategy that demonstrates critical components of the phenol recognition system are not encoded on the Ti plasmid. Conclusions: Although the basic elements of the two-component system required for phenol-mediated induction of virulence gene expression are encoded on the Ti plasmid, they are dependent on the chromosomal background for even the very first stage of signal perception. This discovery suggests a curious evolutionary history, and also provides functional insight into the mechanisms of two-component signal detection and transmission in general.

Original languageEnglish (US)
Pages (from-to)65-76
Number of pages12
JournalChemistry and Biology
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Keywords

  • Signal transduction
  • vir gene induction
  • virA
  • virG
  • Xenognostic phenol recognition

ASJC Scopus subject areas

  • Organic Chemistry

Fingerprint

Dive into the research topics of 'Xenognosin sensing in virulence: Is there a phenol receptor in Agrobacterium tumefaciens?'. Together they form a unique fingerprint.

Cite this