TY - JOUR
T1 - Wnt5a signaling directly affects cell motility and invasion of metastatic melanoma
AU - Weeraratna, Ashani T.
AU - Jiang, Yuan
AU - Hostetter, Galen
AU - Rosenblatt, Kevin
AU - Duray, Paul
AU - Bittner, Michael
AU - Trent, Jeffrey M.
PY - 2002/4
Y1 - 2002/4
N2 - Gene expression profiling identified human melanoma cells demonstrating increased cell motility and invasiveness. The gene WNT5A best determined in vitro invasive behavior. Melanoma cells were transfected with vectors constitutively overexpressing Wnt5a. Consistent changes included actin reorganization and increased cell adhesion. No increase in β-catenin expression or nuclear translocation was observed. There was, however, a dramatic increase in activated PKC. In direct correlation with Wnt5a expression and PKC activation, there was an increase in melanoma cell invasion. Blocking this pathway using antibodies to Frizzled-5, the receptor for Wnt5a, inhibited PKC activity and cellular invasion. Furthermore, Wnt5a expression in human melanoma biopsies directly correlated to increasing tumor grade. These observations support a role for Wnt5a in human melanoma progression.
AB - Gene expression profiling identified human melanoma cells demonstrating increased cell motility and invasiveness. The gene WNT5A best determined in vitro invasive behavior. Melanoma cells were transfected with vectors constitutively overexpressing Wnt5a. Consistent changes included actin reorganization and increased cell adhesion. No increase in β-catenin expression or nuclear translocation was observed. There was, however, a dramatic increase in activated PKC. In direct correlation with Wnt5a expression and PKC activation, there was an increase in melanoma cell invasion. Blocking this pathway using antibodies to Frizzled-5, the receptor for Wnt5a, inhibited PKC activity and cellular invasion. Furthermore, Wnt5a expression in human melanoma biopsies directly correlated to increasing tumor grade. These observations support a role for Wnt5a in human melanoma progression.
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U2 - 10.1016/S1535-6108(02)00045-4
DO - 10.1016/S1535-6108(02)00045-4
M3 - Article
C2 - 12086864
AN - SCOPUS:0036528247
SN - 1535-6108
VL - 1
SP - 279
EP - 288
JO - Cancer cell
JF - Cancer cell
IS - 3
ER -