Wnt signaling pathway analysis in renal cell carcinoma in young patients

Elisabeth Bruder, Holger Moch, David Ehrlich, Ivo Leuschner, Dieter Harms, Pedram Argani, Jakob Briner, Norbert Graf, Barbara Selle, Alex Rufle, Michael Paulussen, Robert Koesters

Research output: Contribution to journalArticle

Abstract

Renal cell carcinomas in young patients constitute a morphologically and genetically heterogeneous group. Twenty percent belong to the newly recognized Xp11.2 translocation-associated family and rare tumors arise from nephroblastoma. Aberrant Wnt signaling through β-catenin mutation has been implicated in nephroblastoma pathogenesis and has been found to synergize with WT1 mutations. To characterize Wnt signaling activity in renal cell carcinomas in young patients, we gathered 34 tumors (three clear cell, ten Xp11.2 translocation associated, five papillary, two chromophobe, two collecting duct, one neuroblastoma associated, eight unclassified renal cell carcinomas, and three carcinomas combined with nephroblastoma) from patients less than 22 years. Expression of β-catenin, its homologue γ-catenin, and of WT1 was assessed by immunohistochemistry in 30 tumors, and sequence analysis of CTNNB1, CTNNG1, and WT1 genes was performed in 25 tumors. Cytoplasmic β-catenin accumulation was demonstrated in two papillary carcinomas, one neuroblastoma-associated carcinoma, and two carcinomas arising from nephroblastoma. The pattern of γ-catenin expression paralleled that of β-catenin but its signal intensity was lower in 22, equal in 7, and stronger only in 1 tumor, respectively. Four tumors showed nuclear WT1 expression. One Xp11.2 translocation-associated carcinoma presented a rare intronic CTNNB1 single nucleotide polymorphism and cytoplasmic β-catenin accumulation. There were no further CTNNB1 or CTNNG1 sequence alterations. A WT1 mutation was found in the nephroblastoma component of a carcinoma arising from nephroblastoma. These findings suggest Wnt signaling pathway activation only in a minority of renal cell carcinomas in young patients. CTNNB1 mutations are rare events. Cytoplasmic β-catenin accumulation in an Xp11.2-associated carcinoma suggests potential interaction of Wnt signaling components with microphthalmia transcription factor family also in Xp11.2 translocation carcinomas. WT1 mutation in the nephroblastoma component of a mixed-type renal cell carcinoma provides direct evidence for clonal independence of nephroblastoma and carcinoma components in this exceptional tumor.

Original languageEnglish (US)
Pages (from-to)1217-1229
Number of pages13
JournalModern Pathology
Volume20
Issue number12
DOIs
StatePublished - Dec 14 2007

Keywords

  • Mutation analysis
  • Pediatric
  • Renal cell carcinoma
  • Wnt pathway

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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  • Cite this

    Bruder, E., Moch, H., Ehrlich, D., Leuschner, I., Harms, D., Argani, P., Briner, J., Graf, N., Selle, B., Rufle, A., Paulussen, M., & Koesters, R. (2007). Wnt signaling pathway analysis in renal cell carcinoma in young patients. Modern Pathology, 20(12), 1217-1229. https://doi.org/10.1038/modpathol.3800957