Wnt signaling controls the stem cell-like asymmetric division of the epithelial seam cells during C. elegans larval development

Julie E. Gleason, David M. Eisenmann

Research output: Contribution to journalArticlepeer-review

Abstract

Metazoan stem cells repopulate tissues during adult life by dividing asymmetrically to generate another stem cell and a cell that terminally differentiates. Wnt signaling regulates the division pattern of stem cells in flies and vertebrates. While the short-lived nematode C. elegans has no adult somatic stem cells, the lateral epithelial seam cells divide in a stem cell-like manner in each larval stage, usually generating a posterior daughter that retains the seam cell fate and an anterior daughter that terminally differentiates. We show that while wild-type adult animals have 16 seam cells per side, animals with reduced function of the TCF homolog POP-1 have as many as 67 seam cells, and animals with reduced function of the β-catenins SYS-1 and WRM-1 have as few as three. Analysis of seam cell division patterns showed alterations in their stem cell-like divisions in the L2-L4 stages: reduced Wnt signaling caused both daughters to adopt non-seam fates, while activated Wnt signaling caused both daughters to adopt the seam fate. Therefore, our results indicate that Wnt signaling globally regulates the asymmetric, stem cell-like division of most or all somatic seam cells during C. elegans larval development, and that Wnt pathway regulation of stem cell-like behavior is conserved in nematodes.

Original languageEnglish (US)
Pages (from-to)58-66
Number of pages9
JournalDevelopmental biology
Volume348
Issue number1
DOIs
StatePublished - Dec 1 2010

Keywords

  • Asymmetric cell division
  • C. elegans
  • Seam cells
  • Stem cells
  • Wnt

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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