WNK4 kinase regulates surface expression of the human sodium chloride cotransporter in mammalian cells

H. Cai, V. Cebotaru, Y. H. Wang, X. M. Zhang, Liudmila Cebotaru, S. E. Guggino, William B Guggino

Research output: Contribution to journalArticle

Abstract

Pseudohypoaldosteronism type II (PHA II) is caused by mutations of two members of WNK ((with no lysine (k)) kinase family. WNK4 wild type (WT) has been shown to inhibit the activity and surface expression of sodium chloride cotransporter (NCC) when expressed in Xenopus oocytes. Here, we have studied NCC protein processing in mammalian cells in the presence or absence of WNK4 WT and its mutants, E562K and R1185C, by surface biotinylation, Western blot, co-immunoprecipitation (Co-IP) and immunostaining. WNK4 WT significantly reduced NCC surface expression in Cos-7 cells (58.9±6.8% vs 100% in control, P

Original languageEnglish (US)
Pages (from-to)2162-2170
Number of pages9
JournalKidney International
Volume69
Issue number12
DOIs
StatePublished - Jun 3 2006

Fingerprint

Sodium Chloride Symporters
Phosphotransferases
Pseudohypoaldosteronism
Biotinylation
Xenopus
Immunoprecipitation
Lysine
Oocytes
Western Blotting
Mutation
Proteins

Keywords

  • Cotransporter
  • Dynamin
  • Lysosomal pathway
  • Pseudohypoaldosteronism type II
  • Sodium chloride
  • WNK4 kinase

ASJC Scopus subject areas

  • Nephrology

Cite this

WNK4 kinase regulates surface expression of the human sodium chloride cotransporter in mammalian cells. / Cai, H.; Cebotaru, V.; Wang, Y. H.; Zhang, X. M.; Cebotaru, Liudmila; Guggino, S. E.; Guggino, William B.

In: Kidney International, Vol. 69, No. 12, 03.06.2006, p. 2162-2170.

Research output: Contribution to journalArticle

Cai, H. ; Cebotaru, V. ; Wang, Y. H. ; Zhang, X. M. ; Cebotaru, Liudmila ; Guggino, S. E. ; Guggino, William B. / WNK4 kinase regulates surface expression of the human sodium chloride cotransporter in mammalian cells. In: Kidney International. 2006 ; Vol. 69, No. 12. pp. 2162-2170.
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AU - Cebotaru, Liudmila

AU - Guggino, S. E.

AU - Guggino, William B

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