Localized temperature control and heater interface remain challenges in centrifugal microfluidics and integrated lab-on-a-chip devices. This paper presents a new wireless heating method that enables selective activation of micropatterned resonant heaters using external radiofrequency (RF) fields and its applications. The wireless heaters in an array are individually activated by modulating the frequency of the external field. Temperature of 93 °C is achieved in the heater when resonated with a 0.49-W RF output power. The wireless method is demonstrated to be fully effective for heating samples under spinning at high speeds, showing its applicability to centrifugal systems. Selective sterilization of Escherichia coli through the wireless heating is also demonstrated. Healthcare applications with a focus on wound sterilization are discussed along with preliminary experiments, showing promising results.