Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields

Vijai Krishnan, Sarah A. Park, Samuel S. Shin, Lina Alon, Caitlin M. Tressler, William Stokes, Jineta Banerjee, Mary E. Sorrell, Yuemin Tian, Gene Y Fridman, Pablo A Celnik, Jonathan A. Pevsner, William B Guggino, Assaf A. Gilad, Galit Pelled

Research output: Contribution to journalArticle

Abstract

The Kryptopterus bicirrhis (glass catfish) is known to respond to electromagnetic fields (EMF). Here we tested its avoidance behavior in response to static and alternating magnetic fields stimulation. Using expression cloning we identified an electromagnetic perceptive gene (EPG) from the K. bicirrhis encoding a protein that responds to EMF. This EPG gene was cloned and expressed in mammalian cells, neuronal cultures and in rat's brain. Immunohistochemistry showed that the expression of EPG is confined to the mammalian cell membrane. Calcium imaging in mammalian cells and cultured neurons expressing EPG demonstrated that remote activation by EMF significantly increases intracellular calcium concentrations, indicative of cellular excitability. Moreover, wireless magnetic activation of EPG in rat motor cortex induced motor evoked responses of the contralateral forelimb in vivo. Here we report on the development of a new technology for remote, non-invasive modulation of cell function.

Original languageEnglish (US)
Article number8764
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Electromagnetic Fields
Electromagnetic Phenomena
Genes
Proteins
Avoidance Learning
Calcium
Catfishes
Forelimb
Motor Cortex
Magnetic Fields
Glass
Organism Cloning
Cultured Cells
Cell Culture Techniques
Immunohistochemistry
Cell Membrane
Technology
Neurons
Brain

ASJC Scopus subject areas

  • General

Cite this

Krishnan, V., Park, S. A., Shin, S. S., Alon, L., Tressler, C. M., Stokes, W., ... Pelled, G. (2018). Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields. Scientific Reports, 8(1), [8764]. https://doi.org/10.1038/s41598-018-27087-9

Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields. / Krishnan, Vijai; Park, Sarah A.; Shin, Samuel S.; Alon, Lina; Tressler, Caitlin M.; Stokes, William; Banerjee, Jineta; Sorrell, Mary E.; Tian, Yuemin; Fridman, Gene Y; Celnik, Pablo A; Pevsner, Jonathan A.; Guggino, William B; Gilad, Assaf A.; Pelled, Galit.

In: Scientific Reports, Vol. 8, No. 1, 8764, 01.12.2018.

Research output: Contribution to journalArticle

Krishnan, V, Park, SA, Shin, SS, Alon, L, Tressler, CM, Stokes, W, Banerjee, J, Sorrell, ME, Tian, Y, Fridman, GY, Celnik, PA, Pevsner, JA, Guggino, WB, Gilad, AA & Pelled, G 2018, 'Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields', Scientific Reports, vol. 8, no. 1, 8764. https://doi.org/10.1038/s41598-018-27087-9
Krishnan, Vijai ; Park, Sarah A. ; Shin, Samuel S. ; Alon, Lina ; Tressler, Caitlin M. ; Stokes, William ; Banerjee, Jineta ; Sorrell, Mary E. ; Tian, Yuemin ; Fridman, Gene Y ; Celnik, Pablo A ; Pevsner, Jonathan A. ; Guggino, William B ; Gilad, Assaf A. ; Pelled, Galit. / Wireless control of cellular function by activation of a novel protein responsive to electromagnetic fields. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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