Wilms tumor locus on 11p13 defined by multiple CpG island-associated transcripts

Laura Bonetta; Stephen E. Kuehn; Annie Huang; David J. Law; Linda M. Kalikin; Minoru Koi; Anthony E. Reeve; Bernard H. Brownstein; Herman Yeger; Bryan R.G. Williams; Andrew P. Feinberg

Wilms tumor locus on 11p13 defined by multiple CpG island-associated transcripts

Laura Bonetta, Stephen E. Kuehn, Annie Huang, David J. Law, Linda M. Kalikin, Minoru Koi, Anthony E. Reeve, Bernard H. Brownstein, Herman Yeger, Bryan R.G. Williams, Andrew P. Feinberg

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

Wilms tumor is an embryonal kidney tumor involving complex pathology and genetics. The Wilms tumor locus on chromosome 11p 13 is defined by the region of overlap of constitutional and tumor-associated deletions. Chromosome walking and yeast artificial chromosome (YAC) cloning were used to clone and map 850 kilobases of DNA. Nine CpG islands, constituting a "CpG island archipelago," were identified, including three islands that were not apparent by conventional pulsed-field mapping, and thus were at least partially methylated. Three distinct transcriptional units were found closely associated with a CpG island within the boundaries of a homozygous DNA deletion in a Wilms tumor.

Original language	English (US)
Pages (from-to)	994-997
Number of pages	4
Journal	Science
Volume	250
Issue number	4983
State	Published - Nov 16 1990
Externally published	Yes

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title = "Wilms tumor locus on 11p13 defined by multiple CpG island-associated transcripts",

abstract = "Wilms tumor is an embryonal kidney tumor involving complex pathology and genetics. The Wilms tumor locus on chromosome 11p 13 is defined by the region of overlap of constitutional and tumor-associated deletions. Chromosome walking and yeast artificial chromosome (YAC) cloning were used to clone and map 850 kilobases of DNA. Nine CpG islands, constituting a {"}CpG island archipelago,{"} were identified, including three islands that were not apparent by conventional pulsed-field mapping, and thus were at least partially methylated. Three distinct transcriptional units were found closely associated with a CpG island within the boundaries of a homozygous DNA deletion in a Wilms tumor.",

author = "Laura Bonetta and Kuehn, {Stephen E.} and Annie Huang and Law, {David J.} and Kalikin, {Linda M.} and Minoru Koi and Reeve, {Anthony E.} and Brownstein, {Bernard H.} and Herman Yeger and Williams, {Bryan R.G.} and Feinberg, {Andrew P.}",

year = "1990",

month = nov,

day = "16",

language = "English (US)",

volume = "250",

pages = "994--997",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

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T1 - Wilms tumor locus on 11p13 defined by multiple CpG island-associated transcripts

AU - Bonetta, Laura

AU - Kuehn, Stephen E.

AU - Huang, Annie

AU - Law, David J.

AU - Kalikin, Linda M.

AU - Koi, Minoru

AU - Reeve, Anthony E.

AU - Brownstein, Bernard H.

AU - Yeger, Herman

AU - Williams, Bryan R.G.

AU - Feinberg, Andrew P.

PY - 1990/11/16

Y1 - 1990/11/16

N2 - Wilms tumor is an embryonal kidney tumor involving complex pathology and genetics. The Wilms tumor locus on chromosome 11p 13 is defined by the region of overlap of constitutional and tumor-associated deletions. Chromosome walking and yeast artificial chromosome (YAC) cloning were used to clone and map 850 kilobases of DNA. Nine CpG islands, constituting a "CpG island archipelago," were identified, including three islands that were not apparent by conventional pulsed-field mapping, and thus were at least partially methylated. Three distinct transcriptional units were found closely associated with a CpG island within the boundaries of a homozygous DNA deletion in a Wilms tumor.

AB - Wilms tumor is an embryonal kidney tumor involving complex pathology and genetics. The Wilms tumor locus on chromosome 11p 13 is defined by the region of overlap of constitutional and tumor-associated deletions. Chromosome walking and yeast artificial chromosome (YAC) cloning were used to clone and map 850 kilobases of DNA. Nine CpG islands, constituting a "CpG island archipelago," were identified, including three islands that were not apparent by conventional pulsed-field mapping, and thus were at least partially methylated. Three distinct transcriptional units were found closely associated with a CpG island within the boundaries of a homozygous DNA deletion in a Wilms tumor.

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AN - SCOPUS:0025695815

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Wilms tumor locus on 11p13 defined by multiple CpG island-associated transcripts

Abstract

ASJC Scopus subject areas

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