Widespread brain distribution of mRNA encoding the orphan neurotransmitter transporter v7-3

K. Inoue, K. Sato, M. Tohyama, S. Shimada, G. R. Uhl

Research output: Contribution to journalArticle

Abstract

Orphan transporter v7-3 is a member of a new subfamily of Na+, Cl- dependent neurotransmitter transporters with two large extracellular loops. Distribution of v7-3 mRNA was investigated in the rat brain. In situ hybridization study revealed that v7-3 mRNA was widely distributed in the rat central nervous system, including the olfactory bulb, the hypothalamus, the cerebral cortex, the hippocampus, and the cerebellum. In addition, intense v7-3 mRNA expression was found in the motor nuclei including the oculomotor nucleus, abducens nucleus, trigeminal motor nucleus, facial nucleus, hypoglossal nucleus and ventral horn of spinal cord. Intense hybridization signals were also observed in the nuclei containing monoaminergic neurons, such as locus coeruleus, the substantia nigra pars compacta, the ventral tegmental area, the dorsal raphe nucleus and the median raphe nucleus. This multifocal and broad nature of the v7-3 distribution suggests widespread roles for this gene product in neurons mediating several important brain function.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalMolecular Brain Research
Volume37
Issue number1-2
DOIs
StatePublished - Apr 1996

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Neurotransmitter Transport Proteins
Messenger RNA
Brain
Neurons
Ventral Tegmental Area
Raphe Nuclei
Locus Coeruleus
Olfactory Bulb
Cerebral Cortex
Cerebellum
Hypothalamus
In Situ Hybridization
Hippocampus
Central Nervous System
Genes

Keywords

  • Hybridization, in situ
  • mRNA expression
  • Sodium- and chloride-dependent neurotransmitter transporter family
  • v7-3
  • Widespread brain distribution

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Widespread brain distribution of mRNA encoding the orphan neurotransmitter transporter v7-3. / Inoue, K.; Sato, K.; Tohyama, M.; Shimada, S.; Uhl, G. R.

In: Molecular Brain Research, Vol. 37, No. 1-2, 04.1996, p. 217-223.

Research output: Contribution to journalArticle

Inoue, K. ; Sato, K. ; Tohyama, M. ; Shimada, S. ; Uhl, G. R. / Widespread brain distribution of mRNA encoding the orphan neurotransmitter transporter v7-3. In: Molecular Brain Research. 1996 ; Vol. 37, No. 1-2. pp. 217-223.
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