Whole-exome sequencing demonstrates recurrent somatic copy number alterations and sporadic mutations in specialized stromal tumors of the prostate

Chin Chen Pan, Toyonori Tsuzuki, Eiichi Morii, Hiroaki Fushimi, Paul Chih Hsueh Chen, Jonathan Ira Epstein

Research output: Contribution to journalArticle

Abstract

In a previous array comparative genomic hybridization study, we detected common deletions of chromosomes 13 and 14 in prostatic stromal sarcoma and stromal tumor of uncertain malignant potential (STUMP). In this study, we performed whole-exome sequencing (WES) and fluorescence in situ hybridization to explore somatic mutations in 1 low-grade stromal sarcoma, 1 high-grade stromal sarcoma, and 12 STUMPs including 5 cases of degenerative atypia type, 1 myxoid type, 1 phyllodes type, and 5 cases of recently described round cell type. WES was successful on 13 cases that revealed frequent somatic copy number alterations including losses of chromosomes 13 (11 cases), 14 (11 cases), and 1p (9 cases), and partial or complete loss of chromosome 10 (7 cases). Fluorescence in situ hybridization was done on 9 cases and showed compatible chromosome 13 copy numbers with the WES results. STUMPs and the low-grade stromal sarcoma carried moderate tumor mutation burdens that ranged from 1.23 to 7.24 mutations per megabase, while the high-grade stromal sarcoma harbored a significantly higher mutation burden (11.55 mutations per megabase). Sporadic somatic mutations were observed, but no recurrent driver mutations could be discerned. In conjunction with prior array comparative genomic hybridization, we have demonstrated the consistent gene dosage profiles that support the clonal nature and the concept of specialized stromal tumors of the prostate as a distinctive tumor entity.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalHuman Pathology
Volume76
DOIs
StatePublished - Jun 1 2018

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Keywords

  • Fluorescence in situ hybridization
  • Prostatic stromal tumor of uncertain malignant potential
  • Somatic copy number alteration
  • Stromal sarcoma
  • Whole-exome sequencing

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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