Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia

A. Gjedde, J. J. Caronna, B. Hindfelt, F. Plum

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The Kety Schmidt washout technique has been modified to measure whole brain blood flow and metabolism in the rat. During nitrous oxide anesthesia, 14 rats exhaled 133Xe, and continuous and simultaneous arterial and cerebral venous samples were drawn from a femoral artery and the transverse sinus of the brain. Extracerebral contamination of the venous sample was minimal, and equilibration of 133Xe in brain tissue and blood was obtained after 10-24 min of inhalation. Cerebral blood flow was calculated from the total activity of the mechanically integrated arterial and venous samples according to the principle of Scheinberg and Stead. At a mean PaCO2 of 40 mmHg, CBF averaged 98 ± 6 (SEM) ml/100 gxmin and CMRO2 averaged 5.4 ± 0.7 (SEM) ml/100 gxmin. CBF changed 2.4% with each millimeter Hg change of PaCO2 while CMRO2 changed only insignificantly. The values obtained for CBF are higher than reported for man and large laboratory animals but reflect the proportionately greater amount of gray matter in the rat brain.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology
Pages113-118
Number of pages6
Volume229
Edition1
StatePublished - 1975
Externally publishedYes

Fingerprint

Nitrous Oxide
Anesthesia
Oxygen
Brain
Cerebrovascular Circulation
Transverse Sinuses
Laboratory Animals
Femoral Artery
Inhalation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Gjedde, A., Caronna, J. J., Hindfelt, B., & Plum, F. (1975). Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia. In American Journal of Physiology (1 ed., Vol. 229, pp. 113-118)

Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia. / Gjedde, A.; Caronna, J. J.; Hindfelt, B.; Plum, F.

American Journal of Physiology. Vol. 229 1. ed. 1975. p. 113-118.

Research output: Chapter in Book/Report/Conference proceedingChapter

Gjedde, A, Caronna, JJ, Hindfelt, B & Plum, F 1975, Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia. in American Journal of Physiology. 1 edn, vol. 229, pp. 113-118.
Gjedde A, Caronna JJ, Hindfelt B, Plum F. Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia. In American Journal of Physiology. 1 ed. Vol. 229. 1975. p. 113-118
Gjedde, A. ; Caronna, J. J. ; Hindfelt, B. ; Plum, F. / Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia. American Journal of Physiology. Vol. 229 1. ed. 1975. pp. 113-118
@inbook{35b0f0eebab54f7690034ecdfd1938a8,
title = "Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia",
abstract = "The Kety Schmidt washout technique has been modified to measure whole brain blood flow and metabolism in the rat. During nitrous oxide anesthesia, 14 rats exhaled 133Xe, and continuous and simultaneous arterial and cerebral venous samples were drawn from a femoral artery and the transverse sinus of the brain. Extracerebral contamination of the venous sample was minimal, and equilibration of 133Xe in brain tissue and blood was obtained after 10-24 min of inhalation. Cerebral blood flow was calculated from the total activity of the mechanically integrated arterial and venous samples according to the principle of Scheinberg and Stead. At a mean PaCO2 of 40 mmHg, CBF averaged 98 ± 6 (SEM) ml/100 gxmin and CMRO2 averaged 5.4 ± 0.7 (SEM) ml/100 gxmin. CBF changed 2.4{\%} with each millimeter Hg change of PaCO2 while CMRO2 changed only insignificantly. The values obtained for CBF are higher than reported for man and large laboratory animals but reflect the proportionately greater amount of gray matter in the rat brain.",
author = "A. Gjedde and Caronna, {J. J.} and B. Hindfelt and F. Plum",
year = "1975",
language = "English (US)",
volume = "229",
pages = "113--118",
booktitle = "American Journal of Physiology",
edition = "1",

}

TY - CHAP

T1 - Whole brain blood flow and oxygen metabolism in the rat during nitrous oxide anesthesia

AU - Gjedde, A.

AU - Caronna, J. J.

AU - Hindfelt, B.

AU - Plum, F.

PY - 1975

Y1 - 1975

N2 - The Kety Schmidt washout technique has been modified to measure whole brain blood flow and metabolism in the rat. During nitrous oxide anesthesia, 14 rats exhaled 133Xe, and continuous and simultaneous arterial and cerebral venous samples were drawn from a femoral artery and the transverse sinus of the brain. Extracerebral contamination of the venous sample was minimal, and equilibration of 133Xe in brain tissue and blood was obtained after 10-24 min of inhalation. Cerebral blood flow was calculated from the total activity of the mechanically integrated arterial and venous samples according to the principle of Scheinberg and Stead. At a mean PaCO2 of 40 mmHg, CBF averaged 98 ± 6 (SEM) ml/100 gxmin and CMRO2 averaged 5.4 ± 0.7 (SEM) ml/100 gxmin. CBF changed 2.4% with each millimeter Hg change of PaCO2 while CMRO2 changed only insignificantly. The values obtained for CBF are higher than reported for man and large laboratory animals but reflect the proportionately greater amount of gray matter in the rat brain.

AB - The Kety Schmidt washout technique has been modified to measure whole brain blood flow and metabolism in the rat. During nitrous oxide anesthesia, 14 rats exhaled 133Xe, and continuous and simultaneous arterial and cerebral venous samples were drawn from a femoral artery and the transverse sinus of the brain. Extracerebral contamination of the venous sample was minimal, and equilibration of 133Xe in brain tissue and blood was obtained after 10-24 min of inhalation. Cerebral blood flow was calculated from the total activity of the mechanically integrated arterial and venous samples according to the principle of Scheinberg and Stead. At a mean PaCO2 of 40 mmHg, CBF averaged 98 ± 6 (SEM) ml/100 gxmin and CMRO2 averaged 5.4 ± 0.7 (SEM) ml/100 gxmin. CBF changed 2.4% with each millimeter Hg change of PaCO2 while CMRO2 changed only insignificantly. The values obtained for CBF are higher than reported for man and large laboratory animals but reflect the proportionately greater amount of gray matter in the rat brain.

UR - http://www.scopus.com/inward/record.url?scp=0016773449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016773449&partnerID=8YFLogxK

M3 - Chapter

C2 - 1147036

AN - SCOPUS:0016773449

VL - 229

SP - 113

EP - 118

BT - American Journal of Physiology

ER -