Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy

Yan Wen, Yan Xing, Lian Chao Yuan, Jian Liu, Ying Zhang, Huan Ying Li

Research output: Contribution to journalArticle

Abstract

We evaluated the sensitivity and specificity of a nested-polymerase chain reaction (PCR) method for detection of Mycobacterium leprae DNA from whole blood. Whole-blood specimens were subjected to nested-PCR amplification of M. leprae repeat DNA sequences in 49 multibacillary (MB) and 30 paucibacillary (PB) leprosy patients, 96 household contacts (HHCs), 18 tuberculosis (TB) patients, and 35 normal healthy individuals. M. leprae DNA was detected in 95.92% (47/49) of MB, 70% (21/30) of PB, and 6.25% (6/96) of HHC, but it was not detected in 18 TB or 35 normal controls. The sensitivities of the anti-bovine serum albumin (ND-O-BSA) immunoglobulin M (IgM) and antifusion protein of ML0405-ML2331 IgG for MB were 97.96% and 89.8%, and these values for PB were 70% and 53.33%. However, the ND-O-BSA enzyme-linked immunosorbent assay (ELISA) had lower specificity, with relatively high false-positive results for TB patients (16.67%) and normal healthy controls (10%). Based on these promising findings, we propose the use of nested PCR of whole-blood samples along with ELISA test for early detection of leprosy cases.

Original languageEnglish (US)
Pages (from-to)918-922
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume88
Issue number5
DOIs
StatePublished - May 2013

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Mycobacterium leprae
Leprosy
Early Diagnosis
Tuberculosis
Polymerase Chain Reaction
DNA
Paucibacillary Leprosy
Enzyme-Linked Immunosorbent Assay
Bovine Serum Albumin
Immunoglobulin M
Immunoglobulin G
Sensitivity and Specificity
Proteins
O-(3,6-di-O-methylglucopyranosyl)-(1-4)-O-(2,3-di-O-methylrhamnopyranosyl)-(1-9)-oxynonanoyl-bovine serum albumin

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy. / Wen, Yan; Xing, Yan; Yuan, Lian Chao; Liu, Jian; Zhang, Ying; Li, Huan Ying.

In: American Journal of Tropical Medicine and Hygiene, Vol. 88, No. 5, 05.2013, p. 918-922.

Research output: Contribution to journalArticle

Wen, Yan ; Xing, Yan ; Yuan, Lian Chao ; Liu, Jian ; Zhang, Ying ; Li, Huan Ying. / Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy. In: American Journal of Tropical Medicine and Hygiene. 2013 ; Vol. 88, No. 5. pp. 918-922.
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