White adipose tissue IFN-γ expression and signalling along the progression of rodent cancer cachexia

Alex Shimura Yamashita, Rodrigo Xavier das Neves, José Cesar Rosa-Neto, Fábio dos Santos Lira, Miguel Luís Batista, Paulo Sérgio Alcantara, José Pinhata Otoch, Marília Seelaender

Research output: Contribution to journalArticlepeer-review

Abstract

Cachexia is associated with increased morbidity and mortality in cancer. The White adipose tissue (WAT) synthesizes and releases several pro-inflammatory cytokines that play a role in cancer cachexia-related systemic inflammation. IFN-γ is a pleiotropic cytokine that regulates several immune and metabolic functions. To assess whether IFN-γ signalling in different WAT pads is modified along cancer-cachexia progression, we evaluated IFN-γ receptors expression (IFNGR1 and IFNGR2) and IFN-γ protein expression in a rodent model of cachexia (7, 10, and 14 days after tumour implantation). IFN-γ protein expression was heterogeneously modulated in WAT, with increases in the mesenteric pad and decreased levels in the retroperitoneal depot along cachexia progression. Ifngr1 was up-regulated 7 days after tumour cell injection in mesenteric and epididymal WAT, but the retroperitoneal depot showed reduced Ifngr1 gene expression. Ifngr2 gene expression was increased 7 and 14 days after tumour inoculation in mesenteric WAT. The results provide evidence that changes in IFN-γ expression and signalling may be perceived at stages preceding refractory cachexia, and therefore, might be employed as a means to assess the early stage of the syndrome.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalCytokine
Volume89
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Keywords

  • Cancer-cachexia
  • Interferon-γ signalling
  • White adipose tissue

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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