When the checkpoints have gone: insights into Cdc25 functional activation.

Seth S. Margolis, Sally Kornbluth

Research output: Contribution to journalArticlepeer-review

Abstract

DNA-responsive checkpoints operate at the G(2)/M transition to prevent premature mitosis in the presence of incompletely replicated or damaged DNA. These pathways prevent mitotic entry, at least in part, by suppressing Cdc25, the phosphatase that activates Cdc2/Cyclin B. To gain insight into how checkpoint signaling controls Cdc25 function, we have carefully examined the individual steps in Cdc25 activation. We found that removal of the regulatory protein, 14-3-3, that binds to phosphorylated Cdc25 during interphase is one of the early steps in mitotic activation. Moreover, our studies unexpectedly implicated the phosphatase PP1 and the G(1)/S kinase Cdk2 in the process of Cdc25 activation. Here we integrate our findings and those of others to propose a model for Cdc25 activation in an effort to provide insight into novel loci of DNA-responsive checkpoint control of mitotic entry.

Original languageEnglish (US)
Pages (from-to)425-428
Number of pages4
JournalCell cycle (Georgetown, Tex.)
Volume3
Issue number4
StatePublished - Apr 2004
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'When the checkpoints have gone: insights into Cdc25 functional activation.'. Together they form a unique fingerprint.

Cite this