What can digital transcript profiling reveal about human cancers?

J. M. Cerutti, G. J. Riggins, Sandro J. de Souza

Research output: Contribution to journalArticle

Abstract

Important biological and clinical features of malignancy are reflected in its transcript pattern. Recent advances in gene expression technology and informatics have provided a powerful new means to obtain and interpret these expression patterns. A comprehensive approach to expression profiling is serial analysis of gene expression (SAGE), which provides digital information on transcript levels. SAGE works by counting transcripts and storing these digital values electronically, providing absolute gene expression levels that make historical comparisons possible. SAGE produces a comprehensive profile of gene expression and can be used to search for candidate tumor markers or antigens in a limited number of samples. The Cancer Genome Anatomy Project has created a SAGE database of human gene expression levels for many different tumors and normal reference tissues and provides online tools for viewing, comparing, and downloading expression profiles. Digital expression profiling using SAGE and informatics have been useful for identifying genes that have a role in tumor invasion and other aspects of tumor progression.

Original languageEnglish (US)
Pages (from-to)975-985
Number of pages11
JournalBrazilian Journal of Medical and Biological Research
Volume36
Issue number8
DOIs
StatePublished - Aug 1 2003

Keywords

  • Cancer
  • Gene expression profiling
  • SAGE
  • Serial analysis of gene expression
  • Tumor antigens

ASJC Scopus subject areas

  • Biophysics
  • Neuroscience(all)
  • Biochemistry
  • Physiology
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cell Biology

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