What are the CD34+ cells in benign peripheral nerve sheath tumors? Double immunostaining study of CD34 and S-100 protein

Mahmoud A. Khalifa, Elizabeth A Montgomery, Nadia Ismiil, Norio Azumi

Research output: Contribution to journalArticle

Abstract

To determine whether CD34 expression in nerve sheath lesions was found in a unique cell population or in a subset of nerve sheath cells, we performed double immunohistochemical staining using a standard avidin-biotin complex method with 2 separate color developing systems. We studied 40 neurofibromas and 16 neurilemomas. All lesions strongly expressed S-100 in nuclei and cytoplasm. CD34 was detected in cells having ameboid dendritic cytoplasm present in greatest numbers in Antoni B zones of neurilemomas, myxoid zones of neurofibromas, at the periphery of lobules in both tumor types, and condensed in apposition to perineurium. The CD34+ cells also were detected in normal nerves. They were infrequent in Antoni A zones of neurilemomas. No dual S-100 and CD34 expression was seen. This double immunostaining confirms the presence of a CD34-reactive non-Schwannian cell type in these neural neoplasms. As the CD34+, S-100-negative cell population is present also in normal nerves and infrequently seen in the areas of cellular neoplastic Schwann cells, CD34+, S-100-negative cells in peripheral nerve sheath tumors most likely are nonneoplastic and may have a supportive function.

Original languageEnglish (US)
Pages (from-to)123-126
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume114
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Nerve Sheath Neoplasms
S100 Proteins
Neurilemmoma
Neurofibroma
Cytoplasm
Avidin
Schwann Cells
Biotin
Peripheral Nerves
Population
Neoplasms
Color
Staining and Labeling
Neurons

Keywords

  • CD34
  • Double immunostaining
  • Neurilemoma
  • Neurofibroma
  • Peripheral nerve sheath tumors
  • S-100
  • Schwannoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

What are the CD34+ cells in benign peripheral nerve sheath tumors? Double immunostaining study of CD34 and S-100 protein. / Khalifa, Mahmoud A.; Montgomery, Elizabeth A; Ismiil, Nadia; Azumi, Norio.

In: American Journal of Clinical Pathology, Vol. 114, No. 1, 2000, p. 123-126.

Research output: Contribution to journalArticle

@article{14393c57c951453aa0cc4d98603d4017,
title = "What are the CD34+ cells in benign peripheral nerve sheath tumors? Double immunostaining study of CD34 and S-100 protein",
abstract = "To determine whether CD34 expression in nerve sheath lesions was found in a unique cell population or in a subset of nerve sheath cells, we performed double immunohistochemical staining using a standard avidin-biotin complex method with 2 separate color developing systems. We studied 40 neurofibromas and 16 neurilemomas. All lesions strongly expressed S-100 in nuclei and cytoplasm. CD34 was detected in cells having ameboid dendritic cytoplasm present in greatest numbers in Antoni B zones of neurilemomas, myxoid zones of neurofibromas, at the periphery of lobules in both tumor types, and condensed in apposition to perineurium. The CD34+ cells also were detected in normal nerves. They were infrequent in Antoni A zones of neurilemomas. No dual S-100 and CD34 expression was seen. This double immunostaining confirms the presence of a CD34-reactive non-Schwannian cell type in these neural neoplasms. As the CD34+, S-100-negative cell population is present also in normal nerves and infrequently seen in the areas of cellular neoplastic Schwann cells, CD34+, S-100-negative cells in peripheral nerve sheath tumors most likely are nonneoplastic and may have a supportive function.",
keywords = "CD34, Double immunostaining, Neurilemoma, Neurofibroma, Peripheral nerve sheath tumors, S-100, Schwannoma",
author = "Khalifa, {Mahmoud A.} and Montgomery, {Elizabeth A} and Nadia Ismiil and Norio Azumi",
year = "2000",
doi = "10.1309/C7N9-J2AU-5TB9-5FRL",
language = "English (US)",
volume = "114",
pages = "123--126",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "1",

}

TY - JOUR

T1 - What are the CD34+ cells in benign peripheral nerve sheath tumors? Double immunostaining study of CD34 and S-100 protein

AU - Khalifa, Mahmoud A.

AU - Montgomery, Elizabeth A

AU - Ismiil, Nadia

AU - Azumi, Norio

PY - 2000

Y1 - 2000

N2 - To determine whether CD34 expression in nerve sheath lesions was found in a unique cell population or in a subset of nerve sheath cells, we performed double immunohistochemical staining using a standard avidin-biotin complex method with 2 separate color developing systems. We studied 40 neurofibromas and 16 neurilemomas. All lesions strongly expressed S-100 in nuclei and cytoplasm. CD34 was detected in cells having ameboid dendritic cytoplasm present in greatest numbers in Antoni B zones of neurilemomas, myxoid zones of neurofibromas, at the periphery of lobules in both tumor types, and condensed in apposition to perineurium. The CD34+ cells also were detected in normal nerves. They were infrequent in Antoni A zones of neurilemomas. No dual S-100 and CD34 expression was seen. This double immunostaining confirms the presence of a CD34-reactive non-Schwannian cell type in these neural neoplasms. As the CD34+, S-100-negative cell population is present also in normal nerves and infrequently seen in the areas of cellular neoplastic Schwann cells, CD34+, S-100-negative cells in peripheral nerve sheath tumors most likely are nonneoplastic and may have a supportive function.

AB - To determine whether CD34 expression in nerve sheath lesions was found in a unique cell population or in a subset of nerve sheath cells, we performed double immunohistochemical staining using a standard avidin-biotin complex method with 2 separate color developing systems. We studied 40 neurofibromas and 16 neurilemomas. All lesions strongly expressed S-100 in nuclei and cytoplasm. CD34 was detected in cells having ameboid dendritic cytoplasm present in greatest numbers in Antoni B zones of neurilemomas, myxoid zones of neurofibromas, at the periphery of lobules in both tumor types, and condensed in apposition to perineurium. The CD34+ cells also were detected in normal nerves. They were infrequent in Antoni A zones of neurilemomas. No dual S-100 and CD34 expression was seen. This double immunostaining confirms the presence of a CD34-reactive non-Schwannian cell type in these neural neoplasms. As the CD34+, S-100-negative cell population is present also in normal nerves and infrequently seen in the areas of cellular neoplastic Schwann cells, CD34+, S-100-negative cells in peripheral nerve sheath tumors most likely are nonneoplastic and may have a supportive function.

KW - CD34

KW - Double immunostaining

KW - Neurilemoma

KW - Neurofibroma

KW - Peripheral nerve sheath tumors

KW - S-100

KW - Schwannoma

UR - http://www.scopus.com/inward/record.url?scp=0034582121&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034582121&partnerID=8YFLogxK

U2 - 10.1309/C7N9-J2AU-5TB9-5FRL

DO - 10.1309/C7N9-J2AU-5TB9-5FRL

M3 - Article

C2 - 10884807

AN - SCOPUS:0034582121

VL - 114

SP - 123

EP - 126

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 1

ER -