What are the CD34+ cells in benign peripheral nerve sheath tumors? Double immunostaining study of CD34 and S-100 protein

Mahmoud A. Khalifa, Elizabeth A. Montgomery, Nadia Ismiil, Norio Azumi

Research output: Contribution to journalArticle

Abstract

To determine whether CD34 expression in nerve sheath lesions was found in a unique cell population or in a subset of nerve sheath cells, we performed double immunohistochemical staining using a standard avidin-biotin complex method with 2 separate color developing systems. We studied 40 neurofibromas and 16 neurilemomas. All lesions strongly expressed S-100 in nuclei and cytoplasm. CD34 was detected in cells having ameboid dendritic cytoplasm present in greatest numbers in Antoni B zones of neurilemomas, myxoid zones of neurofibromas, at the periphery of lobules in both tumor types, and condensed in apposition to perineurium. The CD34+ cells also were detected in normal nerves. They were infrequent in Antoni A zones of neurilemomas. No dual S-100 and CD34 expression was seen. This double immunostaining confirms the presence of a CD34-reactive non-Schwannian cell type in these neural neoplasms. As the CD34+, S-100-negative cell population is present also in normal nerves and infrequently seen in the areas of cellular neoplastic Schwann cells, CD34+, S-100-negative cells in peripheral nerve sheath tumors most likely are nonneoplastic and may have a supportive function.

Original languageEnglish (US)
Pages (from-to)123-126
Number of pages4
JournalAmerican journal of clinical pathology
Volume114
Issue number1
DOIs
StatePublished - Dec 1 2000
Externally publishedYes

Keywords

  • CD34
  • Double immunostaining
  • Neurilemoma
  • Neurofibroma
  • Peripheral nerve sheath tumors
  • S-100
  • Schwannoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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