Wavelet-based analysis of FRET microscopy

Evgeny Kobrinsky, Anna Maltsev, Nikolai M. Soldatov, Darrell R. Abernethy, Donald E. Mager

Research output: Contribution to specialist publicationArticle

Abstract

Förster resonance energy transfer (FRET) microscopy represents a highly useful imaging method for assessing nanometer-scale associations within the living cells. It involves the monitoring of the fluorescence of an acceptor fluorophore when energy is transferred from an excited fluorophore. FRET microscopy can be used to detect protein-protein interactions as well as conformational changes in engineered reporter constructs. A wavelet transform-based approach was proposed to detect and quantify changes in functional cellular microdomains.

Original languageEnglish (US)
Pages49-50
Number of pages2
Volume12
No8
Specialist publicationBiophotonics International
StatePublished - Aug 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology

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