Wavelet analysis reveals heterogeneous time-dependent oscillations of individual mitochondria

Felix T. Kurz, Miguel A. Aon, Brian O'Rourke, Antonis A. Armoundas

Research output: Contribution to journalArticle

Abstract

Mitochondrial inner membrane potential oscillations in cardiac myocytes synchronize under oxidative or metabolic stress, leading to synchronized whole cell oscillations. Gaining information about the temporal properties of individual mitochondrial oscillators is essential to comprehend the network's intrinsic spatiotemporal organization. We have developed methods to detect individual mitochondrial tetramethylrhodamine ethyl ester fluorescence oscillations and assess their dynamical properties using wavelet analysis. We demonstrate that these advanced signal processing tools can provide quantitative spatiotemporal information about intermitochondrial coupling. We have found that the mean frequency of selected groups of continuously oscillating mitochondria was 16.49 ± 1.04 mHz, whereas the mean frequency in the normalized mean global wavelet spectrum was 22.84 ± 1.80 mHz (n = 9 myocytes). In conclusion, this novel methodology will help shed new light on the dynamical properties of the mitochondrial network on the verge of synchronization.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume299
Issue number5
DOIs
StatePublished - Nov 2010

Fingerprint

Wavelet Analysis
Physiological Stress
Mitochondrial Membrane Potential
Cardiac Myocytes
Muscle Cells
Mitochondria
Esters
Oxidative Stress
Fluorescence
tetramethylrhodamine

Keywords

  • Amplitude
  • Frequency
  • Mitochondrial cluster
  • Mitochondrial oscillator

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Wavelet analysis reveals heterogeneous time-dependent oscillations of individual mitochondria. / Kurz, Felix T.; Aon, Miguel A.; O'Rourke, Brian; Armoundas, Antonis A.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 299, No. 5, 11.2010.

Research output: Contribution to journalArticle

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