Waveform modulation of negative-pressure wound therapy in the murine model

Pouya Dastouri, Douglas L. Helm, Saja S. Scherer, Giorgio Pietramaggiori, George Younan, Dennis P. Orgill

Research output: Contribution to journalArticle

Abstract

Background: Negative-pressure wound therapy applied with a porous foam interface has been shown to accelerate granulation-tissue formation when a cyclic application mode of suction is applied, but the optimal waveform has not been determined. The authors hypothesized that changes in the suction waveform applied to wounds would modulate the biological response of granulation tissue formation. Methods: A vacuum-assisted closure device (Kinetic Concepts, Inc., San Antonio, Texas) was applied to full-thickness wounds in 48 male diabetic mice (C57BL/KsJ-Lepr db), which were treated with six different waveforms: square waveforms of 125 mmHg of suction for 2 minutes, alternating with 50 mmHg of suction for 2 minutes, 5 minutes, or 10 minutes; triangular waveform with a 7-minute period oscillating between 50 and 125 mmHg; and static suction at 125 mmHg or static suction at 0 mmHg (occlusive dressing). Wounds were quantitatively evaluated for granulation tissue thickness as well as the number of proliferating cells and the number of blood vessels of the newly formed granulation tissue. Results: At 7 days, the continuous and triangular waveforms induced the thickest granulation tissue, with high rates of cellular proliferation and blood vessel counts compared with square wave and occlusive dressing control wounds. Decreasing square waveform frequency significantly increased granulation tissue thickness, cellular proliferation, and blood vessel counts. Conclusions: Waveform modulation has a significant effect on granulation tissue formation, angiogenesis, and cellular proliferation in excisional wounds in diabetic mice. The rapid change in pressure seen in our square wave model may be detrimental to granulation tissue formation.

Original languageEnglish (US)
Pages (from-to)1460-1466
Number of pages7
JournalPlastic and Reconstructive Surgery
Volume127
Issue number4
DOIs
StatePublished - Apr 1 2011
Externally publishedYes

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Negative-Pressure Wound Therapy
Granulation Tissue
Suction
Occlusive Dressings
Wounds and Injuries
Blood Vessels
Cell Proliferation
Inbred C57BL Mouse
Cell Count
Pressure
Equipment and Supplies

ASJC Scopus subject areas

  • Surgery

Cite this

Dastouri, P., Helm, D. L., Scherer, S. S., Pietramaggiori, G., Younan, G., & Orgill, D. P. (2011). Waveform modulation of negative-pressure wound therapy in the murine model. Plastic and Reconstructive Surgery, 127(4), 1460-1466. https://doi.org/10.1097/PRS.0b013e31820a63cb

Waveform modulation of negative-pressure wound therapy in the murine model. / Dastouri, Pouya; Helm, Douglas L.; Scherer, Saja S.; Pietramaggiori, Giorgio; Younan, George; Orgill, Dennis P.

In: Plastic and Reconstructive Surgery, Vol. 127, No. 4, 01.04.2011, p. 1460-1466.

Research output: Contribution to journalArticle

Dastouri, P, Helm, DL, Scherer, SS, Pietramaggiori, G, Younan, G & Orgill, DP 2011, 'Waveform modulation of negative-pressure wound therapy in the murine model', Plastic and Reconstructive Surgery, vol. 127, no. 4, pp. 1460-1466. https://doi.org/10.1097/PRS.0b013e31820a63cb
Dastouri P, Helm DL, Scherer SS, Pietramaggiori G, Younan G, Orgill DP. Waveform modulation of negative-pressure wound therapy in the murine model. Plastic and Reconstructive Surgery. 2011 Apr 1;127(4):1460-1466. https://doi.org/10.1097/PRS.0b013e31820a63cb
Dastouri, Pouya ; Helm, Douglas L. ; Scherer, Saja S. ; Pietramaggiori, Giorgio ; Younan, George ; Orgill, Dennis P. / Waveform modulation of negative-pressure wound therapy in the murine model. In: Plastic and Reconstructive Surgery. 2011 ; Vol. 127, No. 4. pp. 1460-1466.
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