Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19

Robin McKenzie, Chad K. Porter, Joyce A. Cantrell, Barbara DeNearing, Aisling O'Dowd, Shannon L. Grahek, Stephanie A. Sincock, Colleen Woods, Peter Sebeny, David A. Sack, David R. Tribble, A. Louis Bourgeois, Stephen J. Savarino

Research output: Contribution to journalArticlepeer-review

Abstract

Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 109 organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 109 organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.

Original languageEnglish (US)
Pages (from-to)60-64
Number of pages5
JournalJournal of Infectious Diseases
Volume204
Issue number1
DOIs
StatePublished - Jul 1 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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