Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist Regadenoson

Rebecca W. Pak; Jeeun Kang; Heather Valentine; Leslie M. Loew; Daniel Thorek; Emad M. Boctor; Dean F. Wong; Jin U. Kang

doi:10.1101/316505

Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist Regadenoson

Rebecca W. Pak, Jeeun Kang, Heather Valentine, Leslie M. Loew, Daniel Thorek, Emad M. Boctor, Dean F. Wong, Jin U. Kang

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Optical imaging of brain activity has mostly employed genetically manipulated mice, which cannot be translated to clinical human usage. Observation of brain activity directly is challenging due to difficulty in delivering dyes and other agents through the blood brain barrier (BBB). Using fluorescence imaging, we have demonstrated the feasibility of delivering the near-infrared voltage-sensitive dye (VSD) IR-780 perchlorate to the brain tissue through pharmacological techniques, via an adenosine agonist (Regadenoson). Comparison of VSD fluorescence of mouse brains without and with Regadenoson showed significantly increased residence time of the fluorescence signal in the latter case, indicative of VSD diffusion into the brain tissue. Dose and timing of Regadenoson were varied to optimize BBB permeability for VSD delivery.

Original language	English (US)
Journal	Unknown Journal
DOIs	https://doi.org/10.1101/316505
State	Published - May 7 2018

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Immunology and Microbiology
General Neuroscience
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1101/316505

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title = "Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist Regadenoson",

abstract = "Optical imaging of brain activity has mostly employed genetically manipulated mice, which cannot be translated to clinical human usage. Observation of brain activity directly is challenging due to difficulty in delivering dyes and other agents through the blood brain barrier (BBB). Using fluorescence imaging, we have demonstrated the feasibility of delivering the near-infrared voltage-sensitive dye (VSD) IR-780 perchlorate to the brain tissue through pharmacological techniques, via an adenosine agonist (Regadenoson). Comparison of VSD fluorescence of mouse brains without and with Regadenoson showed significantly increased residence time of the fluorescence signal in the latter case, indicative of VSD diffusion into the brain tissue. Dose and timing of Regadenoson were varied to optimize BBB permeability for VSD delivery.",

author = "Pak, {Rebecca W.} and Jeeun Kang and Heather Valentine and Loew, {Leslie M.} and Daniel Thorek and Boctor, {Emad M.} and Wong, {Dean F.} and Kang, {Jin U.}",

note = "Publisher Copyright: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1101/316505",

language = "English (US)",

journal = "Unknown Journal",

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AU - Pak, Rebecca W.

AU - Kang, Jeeun

AU - Valentine, Heather

AU - Loew, Leslie M.

AU - Thorek, Daniel

AU - Boctor, Emad M.

AU - Wong, Dean F.

AU - Kang, Jin U.

N1 - Publisher Copyright: The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2018/5/7

Y1 - 2018/5/7

N2 - Optical imaging of brain activity has mostly employed genetically manipulated mice, which cannot be translated to clinical human usage. Observation of brain activity directly is challenging due to difficulty in delivering dyes and other agents through the blood brain barrier (BBB). Using fluorescence imaging, we have demonstrated the feasibility of delivering the near-infrared voltage-sensitive dye (VSD) IR-780 perchlorate to the brain tissue through pharmacological techniques, via an adenosine agonist (Regadenoson). Comparison of VSD fluorescence of mouse brains without and with Regadenoson showed significantly increased residence time of the fluorescence signal in the latter case, indicative of VSD diffusion into the brain tissue. Dose and timing of Regadenoson were varied to optimize BBB permeability for VSD delivery.

AB - Optical imaging of brain activity has mostly employed genetically manipulated mice, which cannot be translated to clinical human usage. Observation of brain activity directly is challenging due to difficulty in delivering dyes and other agents through the blood brain barrier (BBB). Using fluorescence imaging, we have demonstrated the feasibility of delivering the near-infrared voltage-sensitive dye (VSD) IR-780 perchlorate to the brain tissue through pharmacological techniques, via an adenosine agonist (Regadenoson). Comparison of VSD fluorescence of mouse brains without and with Regadenoson showed significantly increased residence time of the fluorescence signal in the latter case, indicative of VSD diffusion into the brain tissue. Dose and timing of Regadenoson were varied to optimize BBB permeability for VSD delivery.

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Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist Regadenoson

Abstract

ASJC Scopus subject areas

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