Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist regadenoson

Rebecca W. Pak, Jeeun Kang, Heather Valentine, Leslie M. Loew, Daniel L.J. Thorek, Emad Boctor, Dean Foster Wong, Jin U. Kang

Research output: Contribution to journalArticle

Abstract

Optical imaging of brain activity has mostly employed genetically manipulated mice, which cannot be translated to clinical human usage. Observation of brain activity directly is challenging due to the difficulty in delivering dyes and other agents through the blood brain barrier (BBB). Using fluorescence imaging, we have demonstrated the feasibility of delivering the near-infrared voltage-sensitive dye (VSD) IR-780 perchlorate to the brain tissue through pharmacological techniques, via an adenosine agonist (regadenoson). Comparison of VSD fluorescence of mouse brains without and with regadenoson showed significantly increased residence time of the fluorescence signal in the latter case, indicative of VSD diffusion into the brain tissue. Dose and timing of regadenoson were varied to optimize BBB permeability for VSD delivery.

Original languageEnglish (US)
Article number330308
Pages (from-to)3915-3922
Number of pages8
JournalBiomedical Optics Express
Volume9
Issue number8
DOIs
StatePublished - Aug 1 2018

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Purinergic P1 Receptor Agonists
blood-brain barrier
adenosines
Blood-Brain Barrier
brain
delivery
Coloring Agents
dyes
Brain
electric potential
Optical Imaging
fluorescence
mice
Fluorescence
perchlorates
Adenosine
Permeability
permeability
time measurement
Observation

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Pak, R. W., Kang, J., Valentine, H., Loew, L. M., Thorek, D. L. J., Boctor, E., ... Kang, J. U. (2018). Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist regadenoson. Biomedical Optics Express, 9(8), 3915-3922. [330308]. https://doi.org/10.1364/BOE.9.003915

Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist regadenoson. / Pak, Rebecca W.; Kang, Jeeun; Valentine, Heather; Loew, Leslie M.; Thorek, Daniel L.J.; Boctor, Emad; Wong, Dean Foster; Kang, Jin U.

In: Biomedical Optics Express, Vol. 9, No. 8, 330308, 01.08.2018, p. 3915-3922.

Research output: Contribution to journalArticle

Pak, Rebecca W. ; Kang, Jeeun ; Valentine, Heather ; Loew, Leslie M. ; Thorek, Daniel L.J. ; Boctor, Emad ; Wong, Dean Foster ; Kang, Jin U. / Voltage-sensitive dye delivery through the blood brain barrier using adenosine receptor agonist regadenoson. In: Biomedical Optics Express. 2018 ; Vol. 9, No. 8. pp. 3915-3922.
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