Voltage-gated potassium channels involved in regulation of physiological function in MrgprA3-specific itch neurons

Min Tang, Guanyi Wu, Zhongli Wang, Niuniu Yang, Hao Shi, Qian He, Chan Zhu, Yan Yang, Guang Yu, Changming Wang, Xiaolin Yuan, Qin Liu, Yun Guan, Xinzhong Dong, Zongxiang Tang

Research output: Contribution to journalArticle

Abstract

Itch is described as an unpleasant or irritating skin sensation that elicits the desire or reflex to scratch. MrgprA3, one of members of the Mrgprs family, is specifically expressed in a subpopulation of dorsal root ganglion (DRG) in the peripheral nervous system (PNS). These MrgprA3-expressing DRG neurons have been identified as itch-specific neurons. They can be activated by the compound, chloroquine, which is used as a drug to treat malaria. In the present study, we labeled these itch-specific neurons using the method of molecular genetic markers, and then studied their electrophysiological properties. We also recorded the cutaneous MrgprA3- neurons retrogradely labeled by Dil dye (MrgprA3--Dil). We first found that MrgprA3+ neurons have a lower excitability than MrgprA3- neurons (MrgprA3--non-Dil and MrgprA3--Dil). The number of action potential (AP) was reduced more obviously in MrgprA3+ neurons than that of in MrgprA3- neurons. In most cases, MrgprA3+ neurons only generated single AP; however, in MrgprA3- neurons, the same stimulation could induce multiple AP firing due to the greater voltage-gated potassium (Kv) current existence in MrgprA3+ than in MrgprA3- neurons. Thus, Kv current plays an important role in the regulation of excitability in itch-specific neurons.

Original languageEnglish (US)
Pages (from-to)161-171
Number of pages11
JournalBrain Research
Volume1636
DOIs
StatePublished - Apr 1 2016

Fingerprint

Voltage-Gated Potassium Channels
Neurons
Action Potentials
Spinal Ganglia
Skin
Peripheral Nervous System
Chloroquine
Genetic Markers
Malaria
Reflex
Molecular Biology
Potassium
Coloring Agents

Keywords

  • DRG
  • Itch
  • Kv current
  • MrgprA3

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Voltage-gated potassium channels involved in regulation of physiological function in MrgprA3-specific itch neurons. / Tang, Min; Wu, Guanyi; Wang, Zhongli; Yang, Niuniu; Shi, Hao; He, Qian; Zhu, Chan; Yang, Yan; Yu, Guang; Wang, Changming; Yuan, Xiaolin; Liu, Qin; Guan, Yun; Dong, Xinzhong; Tang, Zongxiang.

In: Brain Research, Vol. 1636, 01.04.2016, p. 161-171.

Research output: Contribution to journalArticle

Tang, M, Wu, G, Wang, Z, Yang, N, Shi, H, He, Q, Zhu, C, Yang, Y, Yu, G, Wang, C, Yuan, X, Liu, Q, Guan, Y, Dong, X & Tang, Z 2016, 'Voltage-gated potassium channels involved in regulation of physiological function in MrgprA3-specific itch neurons', Brain Research, vol. 1636, pp. 161-171. https://doi.org/10.1016/j.brainres.2016.02.014
Tang, Min ; Wu, Guanyi ; Wang, Zhongli ; Yang, Niuniu ; Shi, Hao ; He, Qian ; Zhu, Chan ; Yang, Yan ; Yu, Guang ; Wang, Changming ; Yuan, Xiaolin ; Liu, Qin ; Guan, Yun ; Dong, Xinzhong ; Tang, Zongxiang. / Voltage-gated potassium channels involved in regulation of physiological function in MrgprA3-specific itch neurons. In: Brain Research. 2016 ; Vol. 1636. pp. 161-171.
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AU - He, Qian

AU - Zhu, Chan

AU - Yang, Yan

AU - Yu, Guang

AU - Wang, Changming

AU - Yuan, Xiaolin

AU - Liu, Qin

AU - Guan, Yun

AU - Dong, Xinzhong

AU - Tang, Zongxiang

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AB - Itch is described as an unpleasant or irritating skin sensation that elicits the desire or reflex to scratch. MrgprA3, one of members of the Mrgprs family, is specifically expressed in a subpopulation of dorsal root ganglion (DRG) in the peripheral nervous system (PNS). These MrgprA3-expressing DRG neurons have been identified as itch-specific neurons. They can be activated by the compound, chloroquine, which is used as a drug to treat malaria. In the present study, we labeled these itch-specific neurons using the method of molecular genetic markers, and then studied their electrophysiological properties. We also recorded the cutaneous MrgprA3- neurons retrogradely labeled by Dil dye (MrgprA3--Dil). We first found that MrgprA3+ neurons have a lower excitability than MrgprA3- neurons (MrgprA3--non-Dil and MrgprA3--Dil). The number of action potential (AP) was reduced more obviously in MrgprA3+ neurons than that of in MrgprA3- neurons. In most cases, MrgprA3+ neurons only generated single AP; however, in MrgprA3- neurons, the same stimulation could induce multiple AP firing due to the greater voltage-gated potassium (Kv) current existence in MrgprA3+ than in MrgprA3- neurons. Thus, Kv current plays an important role in the regulation of excitability in itch-specific neurons.

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