Volatile anesthetics and endogenous cannabinoid anandamide have additive and independent inhibitory effects on α7-nicotinic acetylcholine receptor-mediated responses in Xenopus oocytes

Shelley N. Jackson, Sachin K. Singhal, Amina S. Woods, Marisela Morales, Toni Shippenberg, Li Zhang, Murat Oz

Research output: Contribution to journalArticle

Abstract

In earlier studies, the volatile anesthetics and the endogenous cannabinoid anandamide have been shown to inhibit the function of α7-nicotinic acetylcholine receptors. In the present study, interactions between the effects of volatile anesthetics and anandamide on the function of α7-nicotinic acetylcholine receptors expressed in Xenopus oocytes were investigated using the two-electrode voltage-clamp technique. Anandamide and volatile anesthetics isoflurane and halothane inhibited currents evoked with acetylcholine (100 μM) in a reversible and concentration-dependent manner. Coapplication of anandamide and volatile anesthetics caused a significantly greater inhibition of α7-nicotinic acetylcholine receptor function than anandamide or volatile anesthetics alone. Analyses of oocytes by matrix-assisted laser desorption/ionization mass spectroscopy indicated that volatile anesthetics did not alter the lipid profile of oocytes. Results of studies with chimeric α7-nicotinic acetylcholine-5-HT3 receptors comprised of the N-terminal domain of the α7-nicotinic acetylcholine receptor and the transmembrane and carboxyl-terminal domains of 5-HT3 receptors suggest that while isoflurane inhibition of the α7-nicotinic acetylcholine receptor is likely to involve the N-terminal region of the receptor, the site of action for anandamide involves transmembrane and carboxyl-terminal domains of the receptors. These data indicate that endocannabinoids and isoflurane have additive inhibitory effects on α7-nicotinic acetylcholine receptor function through allosteric binding sites located on the distinct regions of the receptor.

Original languageEnglish (US)
Pages (from-to)42-51
Number of pages10
JournalEuropean Journal of Pharmacology
Volume582
Issue number1-3
DOIs
StatePublished - Mar 17 2008
Externally publishedYes

Fingerprint

Cannabinoids
Nicotinic Receptors
Xenopus
Oocytes
Anesthetics
Isoflurane
Receptors, Serotonin, 5-HT3
Acetylcholine
Allosteric Site
Endocannabinoids
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Patch-Clamp Techniques
Halothane
anandamide
Electrodes
Binding Sites
Lipids

Keywords

  • Anandamide
  • Nicotinic receptor
  • Volatile anesthetic
  • Xenopus oocyte

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Volatile anesthetics and endogenous cannabinoid anandamide have additive and independent inhibitory effects on α7-nicotinic acetylcholine receptor-mediated responses in Xenopus oocytes. / Jackson, Shelley N.; Singhal, Sachin K.; Woods, Amina S.; Morales, Marisela; Shippenberg, Toni; Zhang, Li; Oz, Murat.

In: European Journal of Pharmacology, Vol. 582, No. 1-3, 17.03.2008, p. 42-51.

Research output: Contribution to journalArticle

Jackson, Shelley N. ; Singhal, Sachin K. ; Woods, Amina S. ; Morales, Marisela ; Shippenberg, Toni ; Zhang, Li ; Oz, Murat. / Volatile anesthetics and endogenous cannabinoid anandamide have additive and independent inhibitory effects on α7-nicotinic acetylcholine receptor-mediated responses in Xenopus oocytes. In: European Journal of Pharmacology. 2008 ; Vol. 582, No. 1-3. pp. 42-51.
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