Background: Platelet interaction with endothelium plays an important role in the pathophysiology of coronary microcirculation. We assessed the role of the vitronectin receptor (integrin α(v)β3) in platelet/endothelium adhesion. Methods and Results: We investigated the effect on platelet/endothelium adhesion of plasma obtained from patients with acute myocardial infarction during reperfusion (before and 8, 24, 48, and 72 hours and 5 to 7 days after direct angioplasty) and with pretreatment with α- thrombin (2 U/mL)and recombinant human interleukin-1β. Platelet/endothelium adhesion was significantly enhanced by ≃20% after pretreatment of endothelium with patient plasma for 4 hours (P3 on the luminal aspect of endothelium (P3 (platelet glycoprotein IIb-IIIa) to activated endothelial cells was inhibited by antiadhesive peptides GRGDSP and c(RGDfV) and monoclonal antibodies 4F10, LM609, and 7E3. Conclusions: The expression of vitronectin receptor exposed on the luminal aspect of activated endothelium is enhanced and mediates platelet/endothelium adhesion. Vitronectin receptor-mediated platelet attachment to activated endothelium during reperfusion may contribute to reperfusion injury and could be a target for antiadhesive therapy.
|Original language||English (US)|
|Number of pages||10|
|State||Published - Sep 16 1997|
- Myocardial infarction
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine