Vitronectin receptor (α(v)β3) mediates platelet adhesion to the luminal aspect of endothelial cells: Implications for reperfusion in acute myocardial infarction

Meinrad Gawaz, Franz Josef Neumann, Timm Dickfeld, Armin Reininger, Helmut Adelsberger, Adrian Gebhardt, Albert Schömig

Research output: Contribution to journalArticle

Abstract

Background: Platelet interaction with endothelium plays an important role in the pathophysiology of coronary microcirculation. We assessed the role of the vitronectin receptor (integrin α(v)β3) in platelet/endothelium adhesion. Methods and Results: We investigated the effect on platelet/endothelium adhesion of plasma obtained from patients with acute myocardial infarction during reperfusion (before and 8, 24, 48, and 72 hours and 5 to 7 days after direct angioplasty) and with pretreatment with α- thrombin (2 U/mL)and recombinant human interleukin-1β. Platelet/endothelium adhesion was significantly enhanced by ≃20% after pretreatment of endothelium with patient plasma for 4 hours (P3 on the luminal aspect of endothelium (P3 (platelet glycoprotein IIb-IIIa) to activated endothelial cells was inhibited by antiadhesive peptides GRGDSP and c(RGDfV) and monoclonal antibodies 4F10, LM609, and 7E3. Conclusions: The expression of vitronectin receptor exposed on the luminal aspect of activated endothelium is enhanced and mediates platelet/endothelium adhesion. Vitronectin receptor-mediated platelet attachment to activated endothelium during reperfusion may contribute to reperfusion injury and could be a target for antiadhesive therapy.

Original languageEnglish (US)
Pages (from-to)1809-1818
Number of pages10
JournalCirculation
Volume96
Issue number6
StatePublished - Sep 16 1997
Externally publishedYes

Keywords

  • Endothelium
  • Integrins
  • Myocardial infarction
  • Platelets
  • Reperfusion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Gawaz, M., Neumann, F. J., Dickfeld, T., Reininger, A., Adelsberger, H., Gebhardt, A., & Schömig, A. (1997). Vitronectin receptor (α(v)β3) mediates platelet adhesion to the luminal aspect of endothelial cells: Implications for reperfusion in acute myocardial infarction. Circulation, 96(6), 1809-1818.