Abstract
Background: Platelet interaction with endothelium plays an important role in the pathophysiology of coronary microcirculation. We assessed the role of the vitronectin receptor (integrin α(v)β3) in platelet/endothelium adhesion. Methods and Results: We investigated the effect on platelet/endothelium adhesion of plasma obtained from patients with acute myocardial infarction during reperfusion (before and 8, 24, 48, and 72 hours and 5 to 7 days after direct angioplasty) and with pretreatment with α- thrombin (2 U/mL)and recombinant human interleukin-1β. Platelet/endothelium adhesion was significantly enhanced by ≃20% after pretreatment of endothelium with patient plasma for 4 hours (P3 on the luminal aspect of endothelium (P3 (platelet glycoprotein IIb-IIIa) to activated endothelial cells was inhibited by antiadhesive peptides GRGDSP and c(RGDfV) and monoclonal antibodies 4F10, LM609, and 7E3. Conclusions: The expression of vitronectin receptor exposed on the luminal aspect of activated endothelium is enhanced and mediates platelet/endothelium adhesion. Vitronectin receptor-mediated platelet attachment to activated endothelium during reperfusion may contribute to reperfusion injury and could be a target for antiadhesive therapy.
Original language | English (US) |
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Pages (from-to) | 1809-1818 |
Number of pages | 10 |
Journal | Circulation |
Volume | 96 |
Issue number | 6 |
State | Published - Sep 16 1997 |
Externally published | Yes |
Keywords
- Endothelium
- Integrins
- Myocardial infarction
- Platelets
- Reperfusion
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine