Vitrified collagen-based conjunctival equivalent for ocular surface reconstruction

Huifang Zhou, Qiaozhi Lu, Qiongyu Guo, Jemin Chae, Xianqun Fan, Jennifer Hartt Elisseeff, Michael Grant

Research output: Contribution to journalArticle

Abstract

The main functions of the conjunctiva, an essential part of the ocular surface, are to maintain the equilibrium of the tear film and to protect the eye. Upon injuries, the prerequisite to successful ocular surface repair is conjunctival reconstruction. Tissue engineering techniques, including transplantation of autografts, amniotic membranes and numerous synthetic/natural materials, have been developed. However, none of these strategies is completely satisfactory due to lack of goblet cell repopulation, poor mechanical properties or non-standardized preparation procedure. Here, we cultured conjunctival epithelial cells on vitrified collagen membranes and developed a tissue equivalent for repairing damaged conjunctiva. Optimized vitrified collagen has superior mechanical and optical properties to previous biomaterials for ocular surface application, and its unique fibrillar structure significantly benefited conjunctival epithelial cell growth and the phenotypic development invitro. In a rabbit model, vitrified collagen greatly promoted conjunctival regeneration with rapid re-epithelization, sufficient repopulation of goblet cells and minimized fibrosis and wound contracture, proved by gene expression analyses and histological staining. In conclusion, we have demonstrated the potential suitability of utilizing vitrified collagen-based tissue equivalent in ocular surface reconstruction.

Original languageEnglish (US)
Pages (from-to)7398-7406
Number of pages9
JournalBiomaterials
Volume35
Issue number26
DOIs
StatePublished - 2014

Fingerprint

Surface reconstruction
Collagen
Goblet Cells
Conjunctiva
Tissue
Reconstruction (structural)
Transplantation (surgical)
Membranes
Mechanical properties
Epithelial Cells
Autografts
Cell growth
Biocompatible Materials
Tissue engineering
Gene expression
Biomaterials
Amnion
Wounds and Injuries
Contracture
Tissue Engineering

Keywords

  • Conjunctiva
  • Goblet cells
  • Ocular surface
  • Tissue engineering
  • Vitrified collagen

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics
  • Medicine(all)

Cite this

Vitrified collagen-based conjunctival equivalent for ocular surface reconstruction. / Zhou, Huifang; Lu, Qiaozhi; Guo, Qiongyu; Chae, Jemin; Fan, Xianqun; Elisseeff, Jennifer Hartt; Grant, Michael.

In: Biomaterials, Vol. 35, No. 26, 2014, p. 7398-7406.

Research output: Contribution to journalArticle

Zhou, Huifang ; Lu, Qiaozhi ; Guo, Qiongyu ; Chae, Jemin ; Fan, Xianqun ; Elisseeff, Jennifer Hartt ; Grant, Michael. / Vitrified collagen-based conjunctival equivalent for ocular surface reconstruction. In: Biomaterials. 2014 ; Vol. 35, No. 26. pp. 7398-7406.
@article{3d3512f6cfdd4a169524391bf4afcab1,
title = "Vitrified collagen-based conjunctival equivalent for ocular surface reconstruction",
abstract = "The main functions of the conjunctiva, an essential part of the ocular surface, are to maintain the equilibrium of the tear film and to protect the eye. Upon injuries, the prerequisite to successful ocular surface repair is conjunctival reconstruction. Tissue engineering techniques, including transplantation of autografts, amniotic membranes and numerous synthetic/natural materials, have been developed. However, none of these strategies is completely satisfactory due to lack of goblet cell repopulation, poor mechanical properties or non-standardized preparation procedure. Here, we cultured conjunctival epithelial cells on vitrified collagen membranes and developed a tissue equivalent for repairing damaged conjunctiva. Optimized vitrified collagen has superior mechanical and optical properties to previous biomaterials for ocular surface application, and its unique fibrillar structure significantly benefited conjunctival epithelial cell growth and the phenotypic development invitro. In a rabbit model, vitrified collagen greatly promoted conjunctival regeneration with rapid re-epithelization, sufficient repopulation of goblet cells and minimized fibrosis and wound contracture, proved by gene expression analyses and histological staining. In conclusion, we have demonstrated the potential suitability of utilizing vitrified collagen-based tissue equivalent in ocular surface reconstruction.",
keywords = "Conjunctiva, Goblet cells, Ocular surface, Tissue engineering, Vitrified collagen",
author = "Huifang Zhou and Qiaozhi Lu and Qiongyu Guo and Jemin Chae and Xianqun Fan and Elisseeff, {Jennifer Hartt} and Michael Grant",
year = "2014",
doi = "10.1016/j.biomaterials.2014.05.024",
language = "English (US)",
volume = "35",
pages = "7398--7406",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "26",

}

TY - JOUR

T1 - Vitrified collagen-based conjunctival equivalent for ocular surface reconstruction

AU - Zhou, Huifang

AU - Lu, Qiaozhi

AU - Guo, Qiongyu

AU - Chae, Jemin

AU - Fan, Xianqun

AU - Elisseeff, Jennifer Hartt

AU - Grant, Michael

PY - 2014

Y1 - 2014

N2 - The main functions of the conjunctiva, an essential part of the ocular surface, are to maintain the equilibrium of the tear film and to protect the eye. Upon injuries, the prerequisite to successful ocular surface repair is conjunctival reconstruction. Tissue engineering techniques, including transplantation of autografts, amniotic membranes and numerous synthetic/natural materials, have been developed. However, none of these strategies is completely satisfactory due to lack of goblet cell repopulation, poor mechanical properties or non-standardized preparation procedure. Here, we cultured conjunctival epithelial cells on vitrified collagen membranes and developed a tissue equivalent for repairing damaged conjunctiva. Optimized vitrified collagen has superior mechanical and optical properties to previous biomaterials for ocular surface application, and its unique fibrillar structure significantly benefited conjunctival epithelial cell growth and the phenotypic development invitro. In a rabbit model, vitrified collagen greatly promoted conjunctival regeneration with rapid re-epithelization, sufficient repopulation of goblet cells and minimized fibrosis and wound contracture, proved by gene expression analyses and histological staining. In conclusion, we have demonstrated the potential suitability of utilizing vitrified collagen-based tissue equivalent in ocular surface reconstruction.

AB - The main functions of the conjunctiva, an essential part of the ocular surface, are to maintain the equilibrium of the tear film and to protect the eye. Upon injuries, the prerequisite to successful ocular surface repair is conjunctival reconstruction. Tissue engineering techniques, including transplantation of autografts, amniotic membranes and numerous synthetic/natural materials, have been developed. However, none of these strategies is completely satisfactory due to lack of goblet cell repopulation, poor mechanical properties or non-standardized preparation procedure. Here, we cultured conjunctival epithelial cells on vitrified collagen membranes and developed a tissue equivalent for repairing damaged conjunctiva. Optimized vitrified collagen has superior mechanical and optical properties to previous biomaterials for ocular surface application, and its unique fibrillar structure significantly benefited conjunctival epithelial cell growth and the phenotypic development invitro. In a rabbit model, vitrified collagen greatly promoted conjunctival regeneration with rapid re-epithelization, sufficient repopulation of goblet cells and minimized fibrosis and wound contracture, proved by gene expression analyses and histological staining. In conclusion, we have demonstrated the potential suitability of utilizing vitrified collagen-based tissue equivalent in ocular surface reconstruction.

KW - Conjunctiva

KW - Goblet cells

KW - Ocular surface

KW - Tissue engineering

KW - Vitrified collagen

UR - http://www.scopus.com/inward/record.url?scp=84902548819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902548819&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2014.05.024

DO - 10.1016/j.biomaterials.2014.05.024

M3 - Article

C2 - 24933512

AN - SCOPUS:84902548819

VL - 35

SP - 7398

EP - 7406

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 26

ER -