Vitreous Aspirates From Patients with Proliferative Vitreoretinopathy Stimulate Retinal Pigment Epithelial Cell Migration

Peter A Campochiaro, Janice A. Jerdan, Bert M. Glaser, Andrew Cardin, Ronald G. Michels

Research output: Contribution to journalArticle

Abstract

Several studies have focused on retinal pigment epithelial (RPE) cell proliferation as an important event in proliferative vitreoretinopathy (PVR). Little attention has been given to the question of how RPE cells gain access to the vitreous cavity where proliferation occurs. We have recently demonstrated that the serum components fibronectin and platelet-derived growth factor stimulate and direct RPE migration in vitro. In this study, we used this same in vitro technique to examine vitreous aspirates from 13 eyes with PVR, five eyes with macular puckers, and three eyes with uncomplicated retinal detachments for their ability to stimulate RPE migration. We found that aspirates from eyes with PVR stimulated RPE migration to a much greater extent than aspirates from eyes with macular pucker and uncomplicated retinal detachments. The ability to stimulate RPE cell migration correlated with high levels (mean ± SEM, 178 ± 67 mg/L) of immunoreactive fibronectin.

Original languageEnglish (US)
Pages (from-to)1403-1405
Number of pages3
JournalArchives of Ophthalmology
Volume103
Issue number9
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Proliferative Vitreoretinopathy
Retinal Pigments
Cell Movement
Epithelial Cells
Retinal Detachment
Fibronectins
Platelet-Derived Growth Factor
Cell Proliferation
Serum

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Vitreous Aspirates From Patients with Proliferative Vitreoretinopathy Stimulate Retinal Pigment Epithelial Cell Migration. / Campochiaro, Peter A; Jerdan, Janice A.; Glaser, Bert M.; Cardin, Andrew; Michels, Ronald G.

In: Archives of Ophthalmology, Vol. 103, No. 9, 1985, p. 1403-1405.

Research output: Contribution to journalArticle

Campochiaro, Peter A ; Jerdan, Janice A. ; Glaser, Bert M. ; Cardin, Andrew ; Michels, Ronald G. / Vitreous Aspirates From Patients with Proliferative Vitreoretinopathy Stimulate Retinal Pigment Epithelial Cell Migration. In: Archives of Ophthalmology. 1985 ; Vol. 103, No. 9. pp. 1403-1405.
@article{8583af1a226d428fa041cbf8bc76fe11,
title = "Vitreous Aspirates From Patients with Proliferative Vitreoretinopathy Stimulate Retinal Pigment Epithelial Cell Migration",
abstract = "Several studies have focused on retinal pigment epithelial (RPE) cell proliferation as an important event in proliferative vitreoretinopathy (PVR). Little attention has been given to the question of how RPE cells gain access to the vitreous cavity where proliferation occurs. We have recently demonstrated that the serum components fibronectin and platelet-derived growth factor stimulate and direct RPE migration in vitro. In this study, we used this same in vitro technique to examine vitreous aspirates from 13 eyes with PVR, five eyes with macular puckers, and three eyes with uncomplicated retinal detachments for their ability to stimulate RPE migration. We found that aspirates from eyes with PVR stimulated RPE migration to a much greater extent than aspirates from eyes with macular pucker and uncomplicated retinal detachments. The ability to stimulate RPE cell migration correlated with high levels (mean ± SEM, 178 ± 67 mg/L) of immunoreactive fibronectin.",
author = "Campochiaro, {Peter A} and Jerdan, {Janice A.} and Glaser, {Bert M.} and Andrew Cardin and Michels, {Ronald G.}",
year = "1985",
doi = "10.1001/archopht.1985.01050090155053",
language = "English (US)",
volume = "103",
pages = "1403--1405",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "9",

}

TY - JOUR

T1 - Vitreous Aspirates From Patients with Proliferative Vitreoretinopathy Stimulate Retinal Pigment Epithelial Cell Migration

AU - Campochiaro, Peter A

AU - Jerdan, Janice A.

AU - Glaser, Bert M.

AU - Cardin, Andrew

AU - Michels, Ronald G.

PY - 1985

Y1 - 1985

N2 - Several studies have focused on retinal pigment epithelial (RPE) cell proliferation as an important event in proliferative vitreoretinopathy (PVR). Little attention has been given to the question of how RPE cells gain access to the vitreous cavity where proliferation occurs. We have recently demonstrated that the serum components fibronectin and platelet-derived growth factor stimulate and direct RPE migration in vitro. In this study, we used this same in vitro technique to examine vitreous aspirates from 13 eyes with PVR, five eyes with macular puckers, and three eyes with uncomplicated retinal detachments for their ability to stimulate RPE migration. We found that aspirates from eyes with PVR stimulated RPE migration to a much greater extent than aspirates from eyes with macular pucker and uncomplicated retinal detachments. The ability to stimulate RPE cell migration correlated with high levels (mean ± SEM, 178 ± 67 mg/L) of immunoreactive fibronectin.

AB - Several studies have focused on retinal pigment epithelial (RPE) cell proliferation as an important event in proliferative vitreoretinopathy (PVR). Little attention has been given to the question of how RPE cells gain access to the vitreous cavity where proliferation occurs. We have recently demonstrated that the serum components fibronectin and platelet-derived growth factor stimulate and direct RPE migration in vitro. In this study, we used this same in vitro technique to examine vitreous aspirates from 13 eyes with PVR, five eyes with macular puckers, and three eyes with uncomplicated retinal detachments for their ability to stimulate RPE migration. We found that aspirates from eyes with PVR stimulated RPE migration to a much greater extent than aspirates from eyes with macular pucker and uncomplicated retinal detachments. The ability to stimulate RPE cell migration correlated with high levels (mean ± SEM, 178 ± 67 mg/L) of immunoreactive fibronectin.

UR - http://www.scopus.com/inward/record.url?scp=0021930914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021930914&partnerID=8YFLogxK

U2 - 10.1001/archopht.1985.01050090155053

DO - 10.1001/archopht.1985.01050090155053

M3 - Article

C2 - 4038135

AN - SCOPUS:0021930914

VL - 103

SP - 1403

EP - 1405

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 9

ER -