Tracheas from normal and vitamin A-deficient rats were incubated in the presence of l-[3H]fucose and d-[14C]glucosamine to label epithelial and mesenchymal glycoproteins. The epithelium and the cartilage were separated by incubation with testicular hyraluronidase and processed separately for the preparation of glycopeptides. One major epithelial glycopeptide was doubly labeled. In vitamin A deficiency the amount of l-[3H]fucose was reduced to 33% that of the normal. The tracheal fucose-glycopeptide, in molar ratios, contained fucose 1.0, mannonse 1.0, galactose 0.4, hexasamine 4.7, and sialic acid 3.5. Sulfate was absent.
ASJC Scopus subject areas
- Molecular Biology