Prizhiznennoe isledovanie aktivnosti genoma i sinapticheskoi plastichnosti neironov ulitki pri obuchenii.

Translated title of the contribution: Vital investigation of the genome activity and neuronal synaptic plasticity in snail in the course of learning

Alexey Shevelkin, S. A. Kozyrev, V. P. Nikitin, V. V. Sherstnev

Research output: Contribution to journalArticle

Abstract

Vital investigation of genome activity and its role in mechanisms of long-term synaptic plasticity formation were studied in LP11 neuron (command neurone of defence behaviour) during sensitisation (simple form of learning) in semi-intact preparation of snail Helix lucorum. Genome activity was investigate by means of specific fluorescent dye SYTO 16 and image analysis system. It was found that application of sensitizing stimulation (10% quinine solution) onto the snail head initiate two-phase changes in neural responses evoked by tactile or chemical stimulation. Depression of neural responses was obtained during short-term phase (during 1 hour after sensitization) and facilitation of neural responses--during long-term phases (over 1 hour). At the same time (15-20 min after learning), a significant increase in SYTO 16 fluorescent level was found in nucleus site LP11 neurone. Initial SYTO 16 fluorescence level was registered in 4-5 hours after sensitization. If the sensitization was produced during actinomycin D application (inhibitor of RNA synthesis, 20 microM) then facilitation of neural responses evoked by sensory stimulation were suppressed during long-term phase of sensitization and was the same as in control sensitized snails during short-term phase of learning. Increase in SYTO 16 fluorescence level in nucleus region site of LP11 neurone was completely prevented in sensitization during actinomycin D application. If actinomycin D was applied 30 min after sensitization (1 hour after first sensitizing stimulation) then synaptic facilitation and fluorescent dynamics was the same as in control sensitized snails. Our experimental data showed that nociceptive sensitization development was followed by quick (15-20 min) DNA activation and long-term synaptic facilitation (1 hour after sensitization), while induction of the processes was suppressed by inhibitor of RNA synthesis during short time interval (during 1 hour of learning).

Original languageRussian
Pages (from-to)157-168
Number of pages12
JournalRossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk
Volume90
Issue number2
StatePublished - Feb 1 2004
Externally publishedYes

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Neuronal Plasticity
Snails
Dactinomycin
Learning
Genome
Nucleic Acid Synthesis Inhibitors
Neurons
Fluorescence
Helix (Snails)
Chemical Stimulation
Quinine
Touch
Fluorescent Dyes
Head
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Prizhiznennoe isledovanie aktivnosti genoma i sinapticheskoi plastichnosti neironov ulitki pri obuchenii. / Shevelkin, Alexey; Kozyrev, S. A.; Nikitin, V. P.; Sherstnev, V. V.

In: Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova / Rossiiskaia akademiia nauk, Vol. 90, No. 2, 01.02.2004, p. 157-168.

Research output: Contribution to journalArticle

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