Visualizing molecular diffusion through passive permeability barriers in cells: Conventional and novel approaches

Yu Chun Lin, Siew Cheng Phua, Benjamin Lin, Takanari Inoue

Research output: Contribution to journalReview articlepeer-review

Abstract

Diffusion barriers are universal solutions for cells to achieve distinct organizations, compositions, and activities within a limited space. The influence of diffusion barriers on the spatiotemporal dynamics of signaling molecules often determines cellular physiology and functions. Over the years, the passive permeability barriers in various subcellular locales have been characterized using elaborate analytical techniques. In this review, we will summarize the current state of knowledge on the various passive permeability barriers present in mammalian cells. We will conclude with a description of several conventional techniques and one new approach based on chemically inducible diffusion trap (CIDT) for probing permeable barriers.

Original languageEnglish (US)
Pages (from-to)663-671
Number of pages9
JournalCurrent Opinion in Chemical Biology
Volume17
Issue number4
DOIs
StatePublished - Aug 1 2013

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Fingerprint Dive into the research topics of 'Visualizing molecular diffusion through passive permeability barriers in cells: Conventional and novel approaches'. Together they form a unique fingerprint.

Cite this