TY - JOUR
T1 - Visualizing molecular diffusion through passive permeability barriers in cells
T2 - Conventional and novel approaches
AU - Lin, Yu Chun
AU - Phua, Siew Cheng
AU - Lin, Benjamin
AU - Inoue, Takanari
PY - 2013/8
Y1 - 2013/8
N2 - Diffusion barriers are universal solutions for cells to achieve distinct organizations, compositions, and activities within a limited space. The influence of diffusion barriers on the spatiotemporal dynamics of signaling molecules often determines cellular physiology and functions. Over the years, the passive permeability barriers in various subcellular locales have been characterized using elaborate analytical techniques. In this review, we will summarize the current state of knowledge on the various passive permeability barriers present in mammalian cells. We will conclude with a description of several conventional techniques and one new approach based on chemically inducible diffusion trap (CIDT) for probing permeable barriers.
AB - Diffusion barriers are universal solutions for cells to achieve distinct organizations, compositions, and activities within a limited space. The influence of diffusion barriers on the spatiotemporal dynamics of signaling molecules often determines cellular physiology and functions. Over the years, the passive permeability barriers in various subcellular locales have been characterized using elaborate analytical techniques. In this review, we will summarize the current state of knowledge on the various passive permeability barriers present in mammalian cells. We will conclude with a description of several conventional techniques and one new approach based on chemically inducible diffusion trap (CIDT) for probing permeable barriers.
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U2 - 10.1016/j.cbpa.2013.04.027
DO - 10.1016/j.cbpa.2013.04.027
M3 - Review article
C2 - 23731778
AN - SCOPUS:84881223005
SN - 1367-5931
VL - 17
SP - 663
EP - 671
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 4
ER -