Visualization of transcytosis in MDCK cells using laser scanning confocal microscopy

Greg Martin, Rohit Cariappa, Ann L. Hubbard

Research output: Contribution to conferencePaper

Abstract

The targeting signals and delivery routes of DPPIV, a single transmembrane protein whose destination is the apical domain in polarized epithelial cells, is studied. Treatment with Brefeldin A (BFA) resulted in the delivery of DPPIV transfected into Madin-Darby canine cells to the basolateral membrane. After BFA's removal, the molecules are recovered from the basolateral membrane and transcytosed to the apical membrane.

Original languageEnglish (US)
Pages220-221
Number of pages2
StatePublished - Dec 1 1994
EventProceedings of the 52nd Annual Meeting of the Microscopy Society of America - New Orleans, LA, USA
Duration: Jul 31 1994Aug 5 1994

Other

OtherProceedings of the 52nd Annual Meeting of the Microscopy Society of America
CityNew Orleans, LA, USA
Period7/31/948/5/94

ASJC Scopus subject areas

  • Engineering(all)

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    Martin, G., Cariappa, R., & Hubbard, A. L. (1994). Visualization of transcytosis in MDCK cells using laser scanning confocal microscopy. 220-221. Paper presented at Proceedings of the 52nd Annual Meeting of the Microscopy Society of America, New Orleans, LA, USA, .