Visualization of the optic fissure in short-wavelength autofluorescence images of the fundus

Tobias Duncker, Jonathan P. Greenberg, Janet R. Sparrow, R. Theodore Smith, Harry A Quigley, François C. Delori

Research output: Contribution to journalArticle

Abstract

Purpose. To document and explain the presence, inferior to the optic disc, of a distinct vertical boundary between two retinal areas of different short-wavelength autofluorescence (SW-AF) intensities. Methods. SW-AF images of the inferonasal region were acquired from 32 healthy subjects. Additionally, color, 488-nm reflectance (488-R), near-infrared reflectance (NIR-R), NIR autofluorescence (NIR-AF) images, and a spectral domain optical coherence tomography (SD-OCT) image were obtained in selected subjects. Gray levels (GL) on both sides of the demarcation line were measured in SW-AF and 488-R at fixed distances from the disc center. Results. A curved demarcation line inferior to the optic disc was observed on SW-AF images in 31/32 subjects. AF levels on the nasal side were 13% (±6%) lower than on the temporal side at 20° inferior to the disc center. The contrast between the nasal and the temporal areas was not significantly affected by age, sex, refractive error, race, or iris color. The demarcation line visible in SW-AF was also seen, though with reduced contrast, in approximately 80% of the 488-R images (lower reflectance on the nasal side) and 50% of color images. The boundary was not detected by NIR-R, NIR-AF, or by SD-OCT imaging. Conclusions. The location and the distinctness of the demarcation line may indicate a relationship to the closed embryonic optic fissure. The reduced SW-AF intensity and 488-R reflectance observed on the nasal side of this line may be attributable to lower lipofuscin and melanin content per unit area, possibly resulting from a difference in RPE cell shape.

Original languageEnglish (US)
Pages (from-to)6682-6686
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number10
DOIs
StatePublished - Sep 2012

Fingerprint

Nose
Color
Optic Disk
Optical Coherence Tomography
Lipofuscin
Refractive Errors
Cell Shape
Melanins
Iris
Healthy Volunteers

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Visualization of the optic fissure in short-wavelength autofluorescence images of the fundus. / Duncker, Tobias; Greenberg, Jonathan P.; Sparrow, Janet R.; Theodore Smith, R.; Quigley, Harry A; Delori, François C.

In: Investigative Ophthalmology and Visual Science, Vol. 53, No. 10, 09.2012, p. 6682-6686.

Research output: Contribution to journalArticle

Duncker, Tobias ; Greenberg, Jonathan P. ; Sparrow, Janet R. ; Theodore Smith, R. ; Quigley, Harry A ; Delori, François C. / Visualization of the optic fissure in short-wavelength autofluorescence images of the fundus. In: Investigative Ophthalmology and Visual Science. 2012 ; Vol. 53, No. 10. pp. 6682-6686.
@article{107a8b05e30f44f0b90e000869399635,
title = "Visualization of the optic fissure in short-wavelength autofluorescence images of the fundus",
abstract = "Purpose. To document and explain the presence, inferior to the optic disc, of a distinct vertical boundary between two retinal areas of different short-wavelength autofluorescence (SW-AF) intensities. Methods. SW-AF images of the inferonasal region were acquired from 32 healthy subjects. Additionally, color, 488-nm reflectance (488-R), near-infrared reflectance (NIR-R), NIR autofluorescence (NIR-AF) images, and a spectral domain optical coherence tomography (SD-OCT) image were obtained in selected subjects. Gray levels (GL) on both sides of the demarcation line were measured in SW-AF and 488-R at fixed distances from the disc center. Results. A curved demarcation line inferior to the optic disc was observed on SW-AF images in 31/32 subjects. AF levels on the nasal side were 13{\%} (±6{\%}) lower than on the temporal side at 20° inferior to the disc center. The contrast between the nasal and the temporal areas was not significantly affected by age, sex, refractive error, race, or iris color. The demarcation line visible in SW-AF was also seen, though with reduced contrast, in approximately 80{\%} of the 488-R images (lower reflectance on the nasal side) and 50{\%} of color images. The boundary was not detected by NIR-R, NIR-AF, or by SD-OCT imaging. Conclusions. The location and the distinctness of the demarcation line may indicate a relationship to the closed embryonic optic fissure. The reduced SW-AF intensity and 488-R reflectance observed on the nasal side of this line may be attributable to lower lipofuscin and melanin content per unit area, possibly resulting from a difference in RPE cell shape.",
author = "Tobias Duncker and Greenberg, {Jonathan P.} and Sparrow, {Janet R.} and {Theodore Smith}, R. and Quigley, {Harry A} and Delori, {Fran{\cc}ois C.}",
year = "2012",
month = "9",
doi = "10.1167/iovs.12-10004",
language = "English (US)",
volume = "53",
pages = "6682--6686",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "10",

}

TY - JOUR

T1 - Visualization of the optic fissure in short-wavelength autofluorescence images of the fundus

AU - Duncker, Tobias

AU - Greenberg, Jonathan P.

AU - Sparrow, Janet R.

AU - Theodore Smith, R.

AU - Quigley, Harry A

AU - Delori, François C.

PY - 2012/9

Y1 - 2012/9

N2 - Purpose. To document and explain the presence, inferior to the optic disc, of a distinct vertical boundary between two retinal areas of different short-wavelength autofluorescence (SW-AF) intensities. Methods. SW-AF images of the inferonasal region were acquired from 32 healthy subjects. Additionally, color, 488-nm reflectance (488-R), near-infrared reflectance (NIR-R), NIR autofluorescence (NIR-AF) images, and a spectral domain optical coherence tomography (SD-OCT) image were obtained in selected subjects. Gray levels (GL) on both sides of the demarcation line were measured in SW-AF and 488-R at fixed distances from the disc center. Results. A curved demarcation line inferior to the optic disc was observed on SW-AF images in 31/32 subjects. AF levels on the nasal side were 13% (±6%) lower than on the temporal side at 20° inferior to the disc center. The contrast between the nasal and the temporal areas was not significantly affected by age, sex, refractive error, race, or iris color. The demarcation line visible in SW-AF was also seen, though with reduced contrast, in approximately 80% of the 488-R images (lower reflectance on the nasal side) and 50% of color images. The boundary was not detected by NIR-R, NIR-AF, or by SD-OCT imaging. Conclusions. The location and the distinctness of the demarcation line may indicate a relationship to the closed embryonic optic fissure. The reduced SW-AF intensity and 488-R reflectance observed on the nasal side of this line may be attributable to lower lipofuscin and melanin content per unit area, possibly resulting from a difference in RPE cell shape.

AB - Purpose. To document and explain the presence, inferior to the optic disc, of a distinct vertical boundary between two retinal areas of different short-wavelength autofluorescence (SW-AF) intensities. Methods. SW-AF images of the inferonasal region were acquired from 32 healthy subjects. Additionally, color, 488-nm reflectance (488-R), near-infrared reflectance (NIR-R), NIR autofluorescence (NIR-AF) images, and a spectral domain optical coherence tomography (SD-OCT) image were obtained in selected subjects. Gray levels (GL) on both sides of the demarcation line were measured in SW-AF and 488-R at fixed distances from the disc center. Results. A curved demarcation line inferior to the optic disc was observed on SW-AF images in 31/32 subjects. AF levels on the nasal side were 13% (±6%) lower than on the temporal side at 20° inferior to the disc center. The contrast between the nasal and the temporal areas was not significantly affected by age, sex, refractive error, race, or iris color. The demarcation line visible in SW-AF was also seen, though with reduced contrast, in approximately 80% of the 488-R images (lower reflectance on the nasal side) and 50% of color images. The boundary was not detected by NIR-R, NIR-AF, or by SD-OCT imaging. Conclusions. The location and the distinctness of the demarcation line may indicate a relationship to the closed embryonic optic fissure. The reduced SW-AF intensity and 488-R reflectance observed on the nasal side of this line may be attributable to lower lipofuscin and melanin content per unit area, possibly resulting from a difference in RPE cell shape.

UR - http://www.scopus.com/inward/record.url?scp=84871899962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871899962&partnerID=8YFLogxK

U2 - 10.1167/iovs.12-10004

DO - 10.1167/iovs.12-10004

M3 - Article

C2 - 22956617

AN - SCOPUS:84871899962

VL - 53

SP - 6682

EP - 6686

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 10

ER -