Visualization of the inverted terminal repetition in adeno-associated virus DNA

Kenneth I. Berns, Thomas J. Kelly

Research output: Contribution to journalArticle

Abstract

Adeno-associated virus linear, single polynucleotide chains contain an inverted terminal repetition which allows the formation of single-stranded circles when the DNA is exposed to annealing conditions. Under appropriate annealing conditions single-stranded circular dimers are formed, the majority of which have two projections separated by 180 ° visible in the electron microscope. We conclude that these projections represent the regions of self-complementarity (inverted terminal repetition) contained within the virus DNA. Measurements of the lengths of the projections indicate that the length of the inverted terminal repetition represents approximately 1.5% of the genome.

Original languageEnglish (US)
Pages (from-to)267-268,IN9,269-271
JournalJournal of molecular biology
Volume82
Issue number2
DOIs
StatePublished - Jan 15 1974

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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