TY - JOUR
T1 - Visualization of the Hybrid State of tRNA Binding Promoted by Spontaneous Ratcheting of the Ribosome
AU - Agirrezabala, Xabier
AU - Lei, Jianlin
AU - Brunelle, Julie L.
AU - Ortiz-Meoz, Rodrigo F.
AU - Green, Rachel
AU - Frank, Joachim
N1 - Funding Information:
We thank Derek Taylor and Wen Li for discussion of the manuscript, Jayati Sengupta and Ruben L. Gonzalez for valuable suggestions, Robert A. Grassucci for assistance with the image collection, and Michael Watters for assistance with the preparation of the illustrations. This work was supported by Howard Hughes Medical Institute and National Insitutes of Health (NIH) grants R01 GM55440 and R37 GM29169 (to J.F.) and NIH grant R01 GM059425 (to R.G.).
PY - 2008/10/24
Y1 - 2008/10/24
N2 - A crucial step in translation is the translocation of tRNAs through the ribosome. In the transition from one canonical site to the other, the tRNAs acquire intermediate configurations, so-called hybrid states. At this stage, the small subunit is rotated with respect to the large subunit, and the anticodon stem loops reside in the A and P sites of the small subunit, while the acceptor ends interact with the P and E sites of the large subunit. In this work, by means of cryo-EM and particle classification procedures, we visualize the hybrid state of both A/P and P/E tRNAs in an authentic factor-free ribosome complex during translocation. In addition, we show how the repositioning of the tRNAs goes hand in hand with the change in the interplay between S13, L1 stalk, L5, H68, H69, and H38 that is caused by the ratcheting of the small subunit.
AB - A crucial step in translation is the translocation of tRNAs through the ribosome. In the transition from one canonical site to the other, the tRNAs acquire intermediate configurations, so-called hybrid states. At this stage, the small subunit is rotated with respect to the large subunit, and the anticodon stem loops reside in the A and P sites of the small subunit, while the acceptor ends interact with the P and E sites of the large subunit. In this work, by means of cryo-EM and particle classification procedures, we visualize the hybrid state of both A/P and P/E tRNAs in an authentic factor-free ribosome complex during translocation. In addition, we show how the repositioning of the tRNAs goes hand in hand with the change in the interplay between S13, L1 stalk, L5, H68, H69, and H38 that is caused by the ratcheting of the small subunit.
KW - RNA
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U2 - 10.1016/j.molcel.2008.10.001
DO - 10.1016/j.molcel.2008.10.001
M3 - Article
C2 - 18951087
AN - SCOPUS:54049116765
SN - 1097-2765
VL - 32
SP - 190
EP - 197
JO - Molecular cell
JF - Molecular cell
IS - 2
ER -