Visualization of Src Activity at Different Compartments of the Plasma Membrane by FRET Imaging

Jihye Seong, Shaoying Lu, Mingxing Ouyang, He Huang, Jin Zhang, Margaret C. Frame, Yingxiao Wang

Research output: Contribution to journalArticle

Abstract

Membrane compartments function as segregated signaling platforms for different cellular functions. It is not clear how Src is regulated at different membrane compartments. To visualize local Src activity in live cells, a FRET-based Src biosensor was targeted in or outside of lipid rafts at the plasma membrane, via acylation or prenylation modifications on targeting tags either directly fused to the biosensor or coupled to the biosensor through an inducible heterodimerization system. In response to growth factors and pervanadate, the induction of Src activity in rafts was slower and weaker, dependent on actin and possibly its mediated transportation of Src from perinuclear regions to the plasma membrane. In contrast, the induction of Src activity in nonrafts was faster and stronger, dependent on microtubules. Hence, Src activity is differentially regulated via cytoskeleton at different membrane compartments.

Original languageEnglish (US)
Pages (from-to)48-57
Number of pages10
JournalChemistry and Biology
Volume16
Issue number1
DOIs
StatePublished - Jan 30 2009

Keywords

  • CELLBIO
  • CHEMBIO

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Molecular Biology
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology

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