Visualization of multiple FtsZ rings in actively dividing proplastids of cultured Bright Yellow-2 tobacco cells

Yutaka Miyazawa, Toshiyuki Mori, Tamaki Kobayashi, Yu Momoyama, Haruko Kuroiwa, Tsuneyoshi Kuroiwa

Research output: Contribution to journalArticlepeer-review

Abstract

The transfer of stationary-phase cultured tobacco (Nicotiana tabacum L.) Bright Yellow-2 (BY-2) cells into new medium induces active proplastid division. To examine the relationship between ftsZ gene expression and proplastid division, the transcript and protein levels of the ftsZ gene in stationary-phase cells and in 12-h-old cells after medium renewal were compared. RNA-gel blot analysis revealed that transcript levels for both types of ftsZ genes, ftsZ1 and ftsZ2, increased markedly following medium renewal. Similarly, immunoblot analysis using antibodies to lily FtsZ protein showed a remarkable increase in FtsZ protein following medium renewal. Furthermore, immuno-fluorescence microscopic observation revealed that FtsZ proteins appeared as multiple rings transversely aligned in elongated proplastids, which will be fragmented by multiple divisions during culture. These results suggest that FtsZ proteins may have a role in multiple division of proplastids.

Original languageEnglish (US)
Pages (from-to)415-419
Number of pages5
JournalCytologia
Volume66
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Cultured tobacco (Nicotiana tabacum) BY-2 cells
  • FtsZ
  • Proplastid division

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Cell Biology

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