Abstract
Genetically encodable FRET-based kinase activity reporters (KARs) enable real-time monitoring of kinase activity dynamics in living cells with high spatiotemporal resolution. This unit describes a general protocol for utilizing KARs to visualize kinase activity in living mammalian cells with fluorescence microscopy.
Original language | English (US) |
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Journal | Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] |
Volume | Chapter 18 |
DOIs | |
State | Published - Jul 1 2010 |
ASJC Scopus subject areas
- General Medicine