Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy

Xiao Zeng, Shuo Chen, Adam Weitemier, Sanyang Han, Agata Blasiak, Ankshita Prasad, Kezhi Zheng, Zhigao Yi, Baiwen Luo, In Hong Yang, Nitish V Thakor, Chou Chai, Kah Leong Lim, Thomas J. McHugh, Angelo Homayoun All, Xiaogang Liu

Research output: Contribution to journalReview article

Abstract

Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real-time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single-particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra-axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.

Original languageEnglish (US)
Pages (from-to)9262-9268
Number of pages7
JournalAngewandte Chemie - International Edition
Volume58
Issue number27
DOIs
StatePublished - Jul 1 2019

Fingerprint

Microscopic examination
Visualization
Neurodegenerative diseases
Proteins
Imaging techniques
Neurons
Machinery
Nanoparticles
Axons

Keywords

  • axon transport
  • dynein
  • single-particle resolution
  • upconversion microscopy
  • wide-field illumination

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Zeng, X., Chen, S., Weitemier, A., Han, S., Blasiak, A., Prasad, A., ... Liu, X. (2019). Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy. Angewandte Chemie - International Edition, 58(27), 9262-9268. https://doi.org/10.1002/anie.201904208

Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy. / Zeng, Xiao; Chen, Shuo; Weitemier, Adam; Han, Sanyang; Blasiak, Agata; Prasad, Ankshita; Zheng, Kezhi; Yi, Zhigao; Luo, Baiwen; Yang, In Hong; Thakor, Nitish V; Chai, Chou; Lim, Kah Leong; McHugh, Thomas J.; All, Angelo Homayoun; Liu, Xiaogang.

In: Angewandte Chemie - International Edition, Vol. 58, No. 27, 01.07.2019, p. 9262-9268.

Research output: Contribution to journalReview article

Zeng, X, Chen, S, Weitemier, A, Han, S, Blasiak, A, Prasad, A, Zheng, K, Yi, Z, Luo, B, Yang, IH, Thakor, NV, Chai, C, Lim, KL, McHugh, TJ, All, AH & Liu, X 2019, 'Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy', Angewandte Chemie - International Edition, vol. 58, no. 27, pp. 9262-9268. https://doi.org/10.1002/anie.201904208
Zeng, Xiao ; Chen, Shuo ; Weitemier, Adam ; Han, Sanyang ; Blasiak, Agata ; Prasad, Ankshita ; Zheng, Kezhi ; Yi, Zhigao ; Luo, Baiwen ; Yang, In Hong ; Thakor, Nitish V ; Chai, Chou ; Lim, Kah Leong ; McHugh, Thomas J. ; All, Angelo Homayoun ; Liu, Xiaogang. / Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy. In: Angewandte Chemie - International Edition. 2019 ; Vol. 58, No. 27. pp. 9262-9268.
@article{bcda032b3f0b4a3b8ba9ddc64566e4ed,
title = "Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy",
abstract = "Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real-time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single-particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra-axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.",
keywords = "axon transport, dynein, single-particle resolution, upconversion microscopy, wide-field illumination",
author = "Xiao Zeng and Shuo Chen and Adam Weitemier and Sanyang Han and Agata Blasiak and Ankshita Prasad and Kezhi Zheng and Zhigao Yi and Baiwen Luo and Yang, {In Hong} and Thakor, {Nitish V} and Chou Chai and Lim, {Kah Leong} and McHugh, {Thomas J.} and All, {Angelo Homayoun} and Xiaogang Liu",
year = "2019",
month = "7",
day = "1",
doi = "10.1002/anie.201904208",
language = "English (US)",
volume = "58",
pages = "9262--9268",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "27",

}

TY - JOUR

T1 - Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy

AU - Zeng, Xiao

AU - Chen, Shuo

AU - Weitemier, Adam

AU - Han, Sanyang

AU - Blasiak, Agata

AU - Prasad, Ankshita

AU - Zheng, Kezhi

AU - Yi, Zhigao

AU - Luo, Baiwen

AU - Yang, In Hong

AU - Thakor, Nitish V

AU - Chai, Chou

AU - Lim, Kah Leong

AU - McHugh, Thomas J.

AU - All, Angelo Homayoun

AU - Liu, Xiaogang

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real-time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single-particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra-axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.

AB - Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real-time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single-particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra-axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.

KW - axon transport

KW - dynein

KW - single-particle resolution

KW - upconversion microscopy

KW - wide-field illumination

UR - http://www.scopus.com/inward/record.url?scp=85068736691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068736691&partnerID=8YFLogxK

U2 - 10.1002/anie.201904208

DO - 10.1002/anie.201904208

M3 - Review article

C2 - 31087740

AN - SCOPUS:85068736691

VL - 58

SP - 9262

EP - 9268

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 27

ER -