Abstract
Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real-time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single-particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra-axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.
Original language | English (US) |
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Pages (from-to) | 9262-9268 |
Number of pages | 7 |
Journal | Angewandte Chemie - International Edition |
Volume | 58 |
Issue number | 27 |
DOIs | |
State | Published - Jul 1 2019 |
Keywords
- axon transport
- dynein
- single-particle resolution
- upconversion microscopy
- wide-field illumination
ASJC Scopus subject areas
- Catalysis
- General Chemistry