Visualization of chandelier cell axons by parvalbumin immunoreactivity in monkey cerebral cortex

J. DeFelipe, Stewart H Hendry, E. G. Jones

Research output: Contribution to journalArticle

Abstract

Antibodies directed against the calcium-binding protein parvalbumin label a subpopulation of γ-aminobutyric acid-releasing neurons in the cerebral cortex that is thought to have particular metabolic and physiological properties. The chandelier cell is a well-characterized morphological type of γ-aminobutyric acid-releasing cortical interneuron, the axon of which possesses very distinctive terminal portions located around the initial axon segments of pyramidal cells. In the pre- and postcentral gyri of the monkey, we found that these distinctive terminal portions of chandelier cell axons were immuncytochemically stained for parvalbumin in a manner that reveals their complete structure. The chandelier cell axons were identified light-microscopically as short, vertically oriented rows of parvalbumin-positive puncta (PV-Rs). The PV-Rs varied in both length and complexity and were located beneath unstained pyramidal cells. PV-Rs were very numerous in layers II-III, where most pyramidal cells appeared to have a PV-R beneath them. Fewer PV-Rs were found in deeper layers, and in layer VI PV-Rs were rare. With EM all PV-Rs could be seen to form multiple synaptic contacts of the symmetrical type on the initial segments of pyramidal cell axons. Parvalbumin immunoreactivity can therefore be used as a reliable marker for chandelier cell axons.

Original languageEnglish (US)
Pages (from-to)2093-2097
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number6
StatePublished - 1989
Externally publishedYes

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Parvalbumins
Cerebral Cortex
Pyramidal Cells
Haplorhini
Axons
Aminobutyrates
Calcium-Binding Proteins
Somatosensory Cortex
Interneurons
Neurons
Light
Antibodies

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Visualization of chandelier cell axons by parvalbumin immunoreactivity in monkey cerebral cortex",
abstract = "Antibodies directed against the calcium-binding protein parvalbumin label a subpopulation of γ-aminobutyric acid-releasing neurons in the cerebral cortex that is thought to have particular metabolic and physiological properties. The chandelier cell is a well-characterized morphological type of γ-aminobutyric acid-releasing cortical interneuron, the axon of which possesses very distinctive terminal portions located around the initial axon segments of pyramidal cells. In the pre- and postcentral gyri of the monkey, we found that these distinctive terminal portions of chandelier cell axons were immuncytochemically stained for parvalbumin in a manner that reveals their complete structure. The chandelier cell axons were identified light-microscopically as short, vertically oriented rows of parvalbumin-positive puncta (PV-Rs). The PV-Rs varied in both length and complexity and were located beneath unstained pyramidal cells. PV-Rs were very numerous in layers II-III, where most pyramidal cells appeared to have a PV-R beneath them. Fewer PV-Rs were found in deeper layers, and in layer VI PV-Rs were rare. With EM all PV-Rs could be seen to form multiple synaptic contacts of the symmetrical type on the initial segments of pyramidal cell axons. Parvalbumin immunoreactivity can therefore be used as a reliable marker for chandelier cell axons.",
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AU - Hendry, Stewart H

AU - Jones, E. G.

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N2 - Antibodies directed against the calcium-binding protein parvalbumin label a subpopulation of γ-aminobutyric acid-releasing neurons in the cerebral cortex that is thought to have particular metabolic and physiological properties. The chandelier cell is a well-characterized morphological type of γ-aminobutyric acid-releasing cortical interneuron, the axon of which possesses very distinctive terminal portions located around the initial axon segments of pyramidal cells. In the pre- and postcentral gyri of the monkey, we found that these distinctive terminal portions of chandelier cell axons were immuncytochemically stained for parvalbumin in a manner that reveals their complete structure. The chandelier cell axons were identified light-microscopically as short, vertically oriented rows of parvalbumin-positive puncta (PV-Rs). The PV-Rs varied in both length and complexity and were located beneath unstained pyramidal cells. PV-Rs were very numerous in layers II-III, where most pyramidal cells appeared to have a PV-R beneath them. Fewer PV-Rs were found in deeper layers, and in layer VI PV-Rs were rare. With EM all PV-Rs could be seen to form multiple synaptic contacts of the symmetrical type on the initial segments of pyramidal cell axons. Parvalbumin immunoreactivity can therefore be used as a reliable marker for chandelier cell axons.

AB - Antibodies directed against the calcium-binding protein parvalbumin label a subpopulation of γ-aminobutyric acid-releasing neurons in the cerebral cortex that is thought to have particular metabolic and physiological properties. The chandelier cell is a well-characterized morphological type of γ-aminobutyric acid-releasing cortical interneuron, the axon of which possesses very distinctive terminal portions located around the initial axon segments of pyramidal cells. In the pre- and postcentral gyri of the monkey, we found that these distinctive terminal portions of chandelier cell axons were immuncytochemically stained for parvalbumin in a manner that reveals their complete structure. The chandelier cell axons were identified light-microscopically as short, vertically oriented rows of parvalbumin-positive puncta (PV-Rs). The PV-Rs varied in both length and complexity and were located beneath unstained pyramidal cells. PV-Rs were very numerous in layers II-III, where most pyramidal cells appeared to have a PV-R beneath them. Fewer PV-Rs were found in deeper layers, and in layer VI PV-Rs were rare. With EM all PV-Rs could be seen to form multiple synaptic contacts of the symmetrical type on the initial segments of pyramidal cell axons. Parvalbumin immunoreactivity can therefore be used as a reliable marker for chandelier cell axons.

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