Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy

Christian Delamarche, Daniel Thomas, Jean Paul Rolland, Alexandrine Froger, Jean Gouranton, Maria Svelto, Peter C Agre, Giuseppe Calamita

Research output: Contribution to journalArticle

Abstract

Transport of water across the plasma membrane is a fundamental process occurring in all living organisms. In bacteria, osmotic movement of water across the cytoplasmic membrane is needed to maintain cellular turgor; however, the molecular mechanisms of this process are poorly defined. Involvement of aquaporin water channels in bacterial water permeability was suggested by the recent discovery of the aquaporin gene, aqpZ, in Escherichia coli. By employing cryoelectron microscopy to compare E. coli cells containing (AqpZ+) and lacking (AqpZ-) aquaporin, we show that the AqpZ water channel rapidly mediates large water fluxes in response to sudden changes in extracellular osmolarity. These findings (i) demonstrate for the first time functional expression of a prokaryotic water channel, (ii) evidence the bidirectional water channel feature of AqpZ, (iii) document a role for AqpZ in bacterial osmoregulation, and (iv) define a suitable model for studying the physiology of prokaryotic water transport.

Original languageEnglish (US)
Pages (from-to)4193-4197
Number of pages5
JournalJournal of Bacteriology
Volume181
Issue number14
StatePublished - Jul 1999

Fingerprint

Cryoelectron Microscopy
Aquaporins
Permeability
Escherichia coli
Water
Cell Membrane
Water Movements
Osmoregulation
Genetic Association Studies
Osmolar Concentration
Bacteria

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Delamarche, C., Thomas, D., Rolland, J. P., Froger, A., Gouranton, J., Svelto, M., ... Calamita, G. (1999). Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy. Journal of Bacteriology, 181(14), 4193-4197.

Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy. / Delamarche, Christian; Thomas, Daniel; Rolland, Jean Paul; Froger, Alexandrine; Gouranton, Jean; Svelto, Maria; Agre, Peter C; Calamita, Giuseppe.

In: Journal of Bacteriology, Vol. 181, No. 14, 07.1999, p. 4193-4197.

Research output: Contribution to journalArticle

Delamarche, C, Thomas, D, Rolland, JP, Froger, A, Gouranton, J, Svelto, M, Agre, PC & Calamita, G 1999, 'Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy', Journal of Bacteriology, vol. 181, no. 14, pp. 4193-4197.
Delamarche C, Thomas D, Rolland JP, Froger A, Gouranton J, Svelto M et al. Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy. Journal of Bacteriology. 1999 Jul;181(14):4193-4197.
Delamarche, Christian ; Thomas, Daniel ; Rolland, Jean Paul ; Froger, Alexandrine ; Gouranton, Jean ; Svelto, Maria ; Agre, Peter C ; Calamita, Giuseppe. / Visualization of AqpZ-mediated water permeability in Escherichia coli by cryoelectron microscopy. In: Journal of Bacteriology. 1999 ; Vol. 181, No. 14. pp. 4193-4197.
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